PMID- 18397382
OWN - NLM
STAT- MEDLINE
DCOM- 20080924
LR  - 20230425
IS  - 1462-5822 (Electronic)
IS  - 1462-5814 (Print)
IS  - 1462-5814 (Linking)
VI  - 10
IP  - 8
DP  - 2008 Aug
TI  - Nramp1 expression by dendritic cells modulates inflammatory responses during 
      Salmonella Typhimurium infection.
PG  - 1646-61
LID - 10.1111/j.1462-5822.2008.01155.x [doi]
AB  - Host resistance against Salmonella enterica serovar Typhimurium (S. Typhimurium) 
      is mediated by natural resistance-associated macrophage protein 1 
      (Nramp1/Slc11a1). Nramp1 is critical to host defence, as mice lacking Nramp1 fail 
      to control bacterial replication and succumb to low doses of S. Typhimurium. 
      Despite this crucial role, the mechanisms underlying Nramp1's protective effects 
      are unclear. Dendritic cells (DCs) that sample the intestinal lumen are among the 
      first cells encountered by S. Typhimurium following oral infection and act as a 
      conduit for S. Typhimurium to cross the intestinal epithelial barrier. We report 
      that DCs, including intestinal, splenic and bone marrow-derived DCs (BMDCs), 
      express Nramp1 protein. In the small intestine, Nramp1 expression is greater in a 
      subset of DCs (CD11c(+)CD103(-)) characterized by the elevated expression of 
      pro-inflammatory cytokines in response to bacterial products. While Nramp1 
      expression did not affect S. Typhimurium replication in BMDCs, infected Nramp1+/+ 
      BMDCs and intestinal CD11c(+)CD103(-) DCs secreted more inflammatory cytokines 
      (IL-6, IL-12 and TNF-alpha) than Nramp1-/-, suggesting that Nramp1 expression may 
      promote a more rapid inflammatory response following infection. Collectively, 
      these findings reveal a new role for DCs and Nramp1 in modulating the host 
      inflammatory response to S. Typhimurium.
FAU - Valdez, Yanet
AU  - Valdez Y
AD  - Michael Smith Laboratories, University of British Columbia, Vancouver, BC, V6T 
      1Z3, Canada.
FAU - Diehl, Gretchen E
AU  - Diehl GE
FAU - Vallance, Bruce A
AU  - Vallance BA
FAU - Grassl, Guntram A
AU  - Grassl GA
FAU - Guttman, Julian A
AU  - Guttman JA
FAU - Brown, Nat F
AU  - Brown NF
FAU - Rosenberger, Carrie M
AU  - Rosenberger CM
FAU - Littman, Dan R
AU  - Littman DR
FAU - Gros, Philippe
AU  - Gros P
FAU - Finlay, B Brett
AU  - Finlay BB
LA  - eng
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - 5R01 AI033856/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080407
PL  - India
TA  - Cell Microbiol
JT  - Cellular microbiology
JID - 100883691
RN  - 0 (Cation Transport Proteins)
RN  - 0 (Cytokines)
RN  - 0 (natural resistance-associated macrophage protein 1)
SB  - IM
MH  - Animals
MH  - Cation Transport Proteins/*immunology
MH  - Cytokines/immunology
MH  - Dendritic Cells/*immunology
MH  - Intestine, Small/microbiology
MH  - Mice
MH  - Mucous Membrane/immunology
MH  - Salmonella Infections, Animal/*immunology
MH  - Salmonella typhimurium/*immunology
PMC - PMC3051341
MID - NIHMS89629
OID - NLM: HHMIMS89629
EDAT- 2008/04/10 09:00
MHDA- 2008/09/25 09:00
PMCR- 2011/03/09
CRDT- 2008/04/10 09:00
PHST- 2008/04/10 09:00 [pubmed]
PHST- 2008/09/25 09:00 [medline]
PHST- 2008/04/10 09:00 [entrez]
PHST- 2011/03/09 00:00 [pmc-release]
AID - CMI1155 [pii]
AID - 10.1111/j.1462-5822.2008.01155.x [doi]
PST - ppublish
SO  - Cell Microbiol. 2008 Aug;10(8):1646-61. doi: 10.1111/j.1462-5822.2008.01155.x. 
      Epub 2008 Apr 7.