PMID- 18397502
OWN - NLM
STAT- MEDLINE
DCOM- 20080703
LR  - 20160511
IS  - 1865-1674 (Print)
IS  - 1865-1674 (Linking)
VI  - 55
IP  - 2
DP  - 2008
TI  - Effect of natural exposure to vaccine-derived North American genotype PRRS virus 
      on the serological response in naive pigs.
PG  - 140-3
LID - 10.1111/j.1865-1682.2007.01001.x [doi]
AB  - Diagnosis of porcine reproductive and respiratory syndrome virus (PRRSV) is often 
      performed by serological testing, but ELISA does not differentiate between 
      infections with wild-type or vaccine virus. Two attenuated live vaccines 
      [European (EU) or North American (NA) genotype] are used. In addition to 
      wild-type isolates, vaccine or vaccine-derived viruses occur frequently. This is 
      often not considered when the ELISA results are used to differentiate between 
      epizootic and enzootic infections. In this study, an infection with the NA 
      genotype vaccine-derived virus was detected in two herds previously PRRSV 
      negative and ELISA results [sample to positive (s/p) ratios] were analysed. The 
      virus was identified by RT-PCR and nucleotide sequences of ORF5 had 97% (herd A) 
      and 99% (herd B) identity with the genome of a ML PRRSV vaccine belonging to the 
      NA genotype. Pigs of different age became positive with an average s/p ratio of 
      2.24 (A) and 1.18 (B). The data clearly demonstrate that spontaneous infection 
      with a vaccine-derived virus of the NA genotype induces ELISA s/p ratios similar 
      to those induced by vaccination or by infection with wild-type virus. We conclude 
      that for a correct interpretation of serological results the circulation of 
      vaccine or vaccine-derived virus isolates has to be excluded by RT-PCR, even if 
      vaccination is not ongoing.
FAU - Lillie, K
AU  - Lillie K
AD  - Schweinegesundheitsdienst, Schaflandstr 3/3, Fellbach, Germany.
FAU - Igelbrink, R
AU  - Igelbrink R
FAU - Hoferer, M
AU  - Hoferer M
FAU - Fiebig, K
AU  - Fiebig K
FAU - Nathues, H
AU  - Nathues H
FAU - Greiser-Wilke, I
AU  - Greiser-Wilke I
FAU - Grosse Beilage, E
AU  - Grosse Beilage E
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Transbound Emerg Dis
JT  - Transboundary and emerging diseases
JID - 101319538
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/analysis
MH  - DNA, Viral/analysis
MH  - Disease Transmission, Infectious/veterinary
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Female
MH  - Germany/epidemiology
MH  - Male
MH  - Porcine Reproductive and Respiratory Syndrome/blood/epidemiology/*prevention & 
      control/transmission
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Swine
MH  - Vaccination/veterinary
MH  - Viral Vaccines/*immunology
EDAT- 2008/04/10 09:00
MHDA- 2008/07/04 09:00
CRDT- 2008/04/10 09:00
PHST- 2008/04/10 09:00 [pubmed]
PHST- 2008/07/04 09:00 [medline]
PHST- 2008/04/10 09:00 [entrez]
AID - JVA1001 [pii]
AID - 10.1111/j.1865-1682.2007.01001.x [doi]
PST - ppublish
SO  - Transbound Emerg Dis. 2008;55(2):140-3. doi: 10.1111/j.1865-1682.2007.01001.x.