PMID- 18397773
OWN - NLM
STAT- MEDLINE
DCOM- 20080819
LR  - 20080409
IS  - 1389-1723 (Print)
IS  - 1347-4421 (Linking)
VI  - 105
IP  - 3
DP  - 2008 Mar
TI  - The mixed coculture effect of primary rat hepatocytes and bone marrow cells is 
      caused by soluble factors derived from bone marrow cells.
PG  - 226-31
LID - 10.1263/jbb.105.226 [doi]
AB  - Heterospheroids consisting of hepatocytes and bone marrow cells (BMCs) are formed 
      by the mixed coculture of these cells and enhance the expression and maintenance 
      of the liver-specific functions of hepatocytes. Not only the soluble factors 
      derived from these cells, but also functional organoid (heterospheroid) 
      formation, are considered to underlie this coculture effect. Therefore, in the 
      present study, we aimed to clarify the mechanism of this co-culture effect. We 
      performed hepatocyte monoculture with conditioned media prepared from hepatocyte 
      cultures, BMC cultures and a coculture of hepatocytes and BMCs. When using any 
      type of conditioned medium, no hepatocyte spheroids formed, and the hepatocytes 
      formed a monolayer. In addition, an effect for these conditioned media was shown 
      in terms of the albumin production and ammonia metabolism activities of the 
      hepatocytes; conditioned medium from BMCs showed the strongest effect. The 
      monocultured hepatocytes in the conditioned medium derived from BMCs showed 
      equivalent albumin production and ammonia metabolism activities to the cocultured 
      spheroids of hepatocytes and BMCs. Therefore, it was determined that the effect 
      of the coculture of hepatocytes and BMCs was caused by soluble factors derived 
      from BMCs.
FAU - Ijima, Hiroyuki
AU  - Ijima H
AD  - Department of Chemical Engineering, Faculty of Engineering, Graduate School, 
      Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. 
      ijima@chem-eng.kyushu-u.ac.jp
FAU - Matsuo, Takeshi
AU  - Matsuo T
FAU - Kawakami, Koei
AU  - Kawakami K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Biosci Bioeng
JT  - Journal of bioscience and bioengineering
JID - 100888800
RN  - 0 (Biological Factors)
RN  - 0 (Culture Media, Conditioned)
SB  - IM
MH  - Animals
MH  - Biological Factors/*metabolism
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Coculture Techniques
MH  - Culture Media, Conditioned
MH  - Hepatocytes/*cytology/metabolism
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Spheroids, Cellular/cytology
EDAT- 2008/04/10 09:00
MHDA- 2008/08/20 09:00
CRDT- 2008/04/10 09:00
PHST- 2007/10/30 00:00 [received]
PHST- 2007/12/13 00:00 [accepted]
PHST- 2008/04/10 09:00 [pubmed]
PHST- 2008/08/20 09:00 [medline]
PHST- 2008/04/10 09:00 [entrez]
AID - S1389-1723(08)70057-4 [pii]
AID - 10.1263/jbb.105.226 [doi]
PST - ppublish
SO  - J Biosci Bioeng. 2008 Mar;105(3):226-31. doi: 10.1263/jbb.105.226.