PMID- 18397777
OWN - NLM
STAT- MEDLINE
DCOM- 20080819
LR  - 20080409
IS  - 1389-1723 (Print)
IS  - 1347-4421 (Linking)
VI  - 105
IP  - 3
DP  - 2008 Mar
TI  - Quantitative analysis of anionic metabolites for Catharanthus roseus by capillary 
      electrophoresis using sulfonated capillary coupled with electrospray 
      ionization-tandem mass spectrometry.
PG  - 249-60
LID - 10.1263/jbb.105.249 [doi]
AB  - This paper describes a practical method to quantify anionic metabolites using 
      contemporary capillary electrophoresis-electrospray ionization-tandem mass 
      spectrometry (CE-ESI-MS/MS). The use of sulfonated capillary permitted a 
      simultaneous analysis of sugar phosphates, organic acids, nucleotides and 
      coenzyme A compounds with only one CE condition. This capillary also improved the 
      repeatability and sensitivity. MS/MS with multiple reaction monitoring (MRM) 
      detection was utilized to achieve significant selectivity and sensitivity. Under 
      optimized CE-ESI-MS/MS system, the detection limits of 53 metabolites at 
      signal-to-noise ratio of 3 were between 0.040 and 8.8 mumol/l. The relative 
      standard deviations (RSDs) for the majority anionic metabolites were better than 
      0.5% for migration times, and better than 10.0% for peak areas (n=6). Matrix 
      effects by contaminants in sample solution in CE-ESI-MS/MS analysis were removed 
      dramatically by the sample preparation method with liquid-liquid fractionation 
      and ultrafiltration procedure. Furthermore, the developed method was successfully 
      applied to determine anionic metabolites in a cultured cell of Catharanthus 
      roseus. Accumulation of some metabolites including shikimate, malate, and 
      sedoheptulose 7-phosphate by elicitation of methyl jasmonate was observed. The 
      result would show shikimate, tricarboxylic acid and pentose phosphate pathways 
      were activated. Our method will be useful for detailed analysis of primary 
      metabolism dynamics.
FAU - Harada, Kazuo
AU  - Harada K
AD  - Department of Biotechnology, Graduate School of Engineering, Osaka University, 
      2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
FAU - Ohyama, Yohko
AU  - Ohyama Y
FAU - Tabushi, Tetsuya
AU  - Tabushi T
FAU - Kobayashi, Akio
AU  - Kobayashi A
FAU - Fukusaki, Eiichiro
AU  - Fukusaki E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Biosci Bioeng
JT  - Journal of bioscience and bioengineering
JID - 100888800
RN  - 0 (Anions)
SB  - IM
MH  - Anions
MH  - Catharanthus/*metabolism
MH  - Electrophoresis, Capillary
MH  - Spectrometry, Mass, Electrospray Ionization
EDAT- 2008/04/10 09:00
MHDA- 2008/08/20 09:00
CRDT- 2008/04/10 09:00
PHST- 2007/11/02 00:00 [received]
PHST- 2007/12/19 00:00 [accepted]
PHST- 2008/04/10 09:00 [pubmed]
PHST- 2008/08/20 09:00 [medline]
PHST- 2008/04/10 09:00 [entrez]
AID - S1389-1723(08)70061-6 [pii]
AID - 10.1263/jbb.105.249 [doi]
PST - ppublish
SO  - J Biosci Bioeng. 2008 Mar;105(3):249-60. doi: 10.1263/jbb.105.249.