PMID- 18398014
OWN - NLM
STAT- MEDLINE
DCOM- 20090220
LR  - 20191210
IS  - 1460-2385 (Electronic)
IS  - 0931-0509 (Linking)
VI  - 23
IP  - 10
DP  - 2008 Oct
TI  - Utilization of nanobiotechnology in haemodialysis: mock-dialysis experiments on 
      homocysteine.
PG  - 3234-9
LID - 10.1093/ndt/gfn189 [doi]
AB  - BACKGROUND: The utilization of modern achievements from nanobiotechnology has 
      resulted in novel modalities for renal replacement therapy. For conventional 
      intermittent haemodialysis (HD), sophisticated membranes are currently being 
      manufactured that guarantee selective removal of target toxins. These membranes 
      have a narrow pore-size distribution that is focused around a mean value at the 
      nanometre level. For continuous HD, novel artificial renal devices are currently 
      being designed and evaluated in in vitro experiments that will be both 
      implantable and have continuous function. METHODS: We present mock-dialysis 
      experiments using magnetically assisted HD (MAHD) that we very recently 
      introduced for the selective removal of target toxins. MAHD is based on the 
      preparation of conjugates (Cs) made up of biocompatible ferromagnetic 
      nanoparticles (FNs) and a specifically designed targeted binding substance that 
      must have a high affinity for a specific target toxin substance. The FN-targeted 
      binding substance Cs should be administered to the patient prior to MAHD to allow 
      for binding with the target toxin substance in the bloodstream. The complex 
      FN-targeted binding substance-target toxin substance will then be removed by a 
      'magnetic dialyzer' that is installed in the dialysis machine in series to the 
      conventional dialyzer. In the present work, we compared the in vitro efficiency 
      of MAHD to conventional HD for the removal of homocysteine (Hcy) during 
      mock-dialysis experiments. RESULTS: These mock-dialysis experiments performed on 
      Hcy revealed that both the removal rate and the overall removal efficiency of 
      MAHD were significantly greater than conventional HD. CONCLUSIONS: MAHD appears 
      to be a promising method that can be employed for the selective and more 
      efficient extraction of toxins that are not adequately removed by conventional 
      HD.
FAU - Stamopoulos, Dimosthenis
AU  - Stamopoulos D
AD  - Institute of Materials Science, NCSR Demokritos, Aghia Paraskevi 153-10, Athens, 
      Greece. densta@ims.demokritos.gr
FAU - Bouziotis, Penelope
AU  - Bouziotis P
FAU - Benaki, Dimitra
AU  - Benaki D
FAU - Kotsovassilis, Constantinos
AU  - Kotsovassilis C
FAU - Zirogiannis, Panagiotis N
AU  - Zirogiannis PN
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
DEP - 20080408
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Ferric Compounds)
RN  - 0 (Toxins, Biological)
RN  - 0 (uremia middle molecule toxins)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 1317-54-0 (ferrite)
RN  - 27432CM55Q (Serum Albumin, Bovine)
SB  - IM
MH  - Animals
MH  - Biotechnology
MH  - Cattle
MH  - Circular Dichroism
MH  - Ferric Compounds
MH  - Homocysteine/blood/*isolation & purification
MH  - Humans
MH  - In Vitro Techniques
MH  - Magnetics
MH  - Metal Nanoparticles
MH  - Microscopy, Atomic Force
MH  - *Nanotechnology
MH  - Renal Dialysis/*methods
MH  - Serum Albumin, Bovine
MH  - Toxins, Biological/blood/isolation & purification
EDAT- 2008/04/10 09:00
MHDA- 2009/02/21 09:00
CRDT- 2008/04/10 09:00
PHST- 2008/04/10 09:00 [pubmed]
PHST- 2009/02/21 09:00 [medline]
PHST- 2008/04/10 09:00 [entrez]
AID - gfn189 [pii]
AID - 10.1093/ndt/gfn189 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2008 Oct;23(10):3234-9. doi: 10.1093/ndt/gfn189. Epub 
      2008 Apr 8.