PMID- 18398665
OWN - NLM
STAT- MEDLINE
DCOM- 20080926
LR  - 20211020
IS  - 1071-2690 (Print)
IS  - 1071-2690 (Linking)
VI  - 44
IP  - 5-6
DP  - 2008 May-Jun
TI  - Nestin-positive spheres derived from canine bone marrow stromal cells generate 
      cells with early neuronal and glial phenotypic characteristics.
PG  - 140-4
LID - 10.1007/s11626-008-9089-x [doi]
AB  - Bone marrow stromal cells (BMSCs) isolated from humans and rodents have been 
      shown to generate neural cells under specific culture conditions and after 
      transplantation in the central nervous system. The apparent plasticity of BMSCs 
      has therefore been a target of intensive research in attempt to develop a novel 
      therapy for neurological diseases. Canines sustain neurological disorders (e.g., 
      traumatic spinal cord injury) that closely mirror pathology of those in humans. 
      Therefore, we evaluated neural differentiation properties of canine BMSCs to 
      provide insights into basic characterization of these cells for future 
      neurotransplantation trials in canine patients with neurological disorders. We 
      demonstrate that canine BMSCs form spherical cellular aggregates on anti-adhesive 
      culture substrate in serum-free culture media, which morphologically and 
      phenotypically resemble spherical aggregates of neural progenitor cells, 
      so-called neurospheres. Upon dissociation and subculture on adhesive substrate, 
      canine BMSCs express neuronal (ss capital SHA, Cyrillic-tubulin) and glial (GFAP, 
      A2B5, and CNPase) markers. Formation of spherical aggregates appears to be a 
      critical preceding process for some of the glial marker expression (CNPase and 
      A2B5). However, expression of more mature neuronal (MAP2) and glial (MBP) markers 
      could not be induced with the protocol used in this study. We suggest that 
      induction of canine BMSCs into cells with neural progenitor cell characteristics 
      is possible and that these cells may have the potential for future cellular 
      therapy for neurological disorders.
FAU - Kamishina, Hiroaki
AU  - Kamishina H
AD  - Department of Small Animal Clinical Sciences, College of Veterinary Medicine, 
      University of Florida, Gainesville, FL 32610-0126, USA. Kamicna@iwate-u.ac.jp
FAU - Cheeseman, Jennifer A
AU  - Cheeseman JA
FAU - Clemmons, Roger M
AU  - Clemmons RM
LA  - eng
PT  - Journal Article
DEP - 20080409
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
RN  - 0 (Biomarkers)
RN  - 0 (Intermediate Filament Proteins)
RN  - 0 (NES protein, human)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nestin)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/*cytology
MH  - Cells, Cultured
MH  - Dogs
MH  - Intermediate Filament Proteins/*metabolism
MH  - Nerve Tissue Proteins/*metabolism
MH  - Nestin
MH  - Neuroglia/*cytology
MH  - Neurons/*cytology
MH  - Phenotype
MH  - Stromal Cells/*cytology
EDAT- 2008/04/10 09:00
MHDA- 2008/09/27 09:00
CRDT- 2008/04/10 09:00
PHST- 2007/11/13 00:00 [received]
PHST- 2008/02/15 00:00 [accepted]
PHST- 2008/04/10 09:00 [pubmed]
PHST- 2008/09/27 09:00 [medline]
PHST- 2008/04/10 09:00 [entrez]
AID - 10.1007/s11626-008-9089-x [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 2008 May-Jun;44(5-6):140-4. doi: 
      10.1007/s11626-008-9089-x. Epub 2008 Apr 9.