PMID- 18400865
OWN - NLM
STAT- MEDLINE
DCOM- 20080812
LR  - 20211020
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 82
IP  - 12
DP  - 2008 Jun
TI  - Site 1 protease is required for proteolytic processing of the glycoproteins of 
      the South American hemorrhagic fever viruses Junin, Machupo, and Guanarito.
PG  - 6045-51
LID - 10.1128/JVI.02392-07 [doi]
AB  - The cellular proprotein convertase site 1 protease (S1P) has been implicated in 
      the proteolytic processing of the glycoproteins (GPs) of Old World arenaviruses. 
      Here we report that S1P is also involved in the processing of the GPs of the 
      genetically more-distant South American hemorrhagic fever viruses Guanarito, 
      Machupo, and Junin. Efficient cleavage of Guanarito virus GP, whose protease 
      recognition sites deviate from the reported S1P consensus sequence, indicates a 
      broader specificity of S1P than anticipated. Lack of GP processing of Junin virus 
      dramatically reduced production of infectious virus and prevented cell-to-cell 
      propagation. Infection of S1P-deficient cells resulted in viral persistence over 
      several weeks without the emergence of escape variants able to use other cellular 
      proteases for GP processing.
FAU - Rojek, Jillian M
AU  - Rojek JM
AD  - Molecular and Integrative Neurosciences Department, The Scripps Research 
      Institute, La Jolla, California 92037, USA.
FAU - Lee, Andrew M
AU  - Lee AM
FAU - Nguyen, NgocThao
AU  - Nguyen N
FAU - Spiropoulou, Christina F
AU  - Spiropoulou CF
FAU - Kunz, Stefan
AU  - Kunz S
LA  - eng
GR  - R21 AI065560/AI/NIAID NIH HHS/United States
GR  - U54 AI065359/AI/NIAID NIH HHS/United States
GR  - 1U54 AI065359/AI/NIAID NIH HHS/United States
GR  - AI065560/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080409
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Glycoproteins)
RN  - 0 (Protein Precursors)
RN  - 0 (Recombinant Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 3.4.21.- (Proprotein Convertases)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.112 (membrane-bound transcription factor peptidase, site 1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Arenaviruses, New World/*metabolism
MH  - CHO Cells
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Cricetinae
MH  - Cricetulus
MH  - Glycoproteins/*metabolism
MH  - Green Fluorescent Proteins/metabolism
MH  - Humans
MH  - Kidney/cytology
MH  - Luciferases/analysis/metabolism
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Proprotein Convertases/chemistry/genetics/*metabolism
MH  - Protein Precursors/chemistry/metabolism
MH  - *Protein Processing, Post-Translational
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Serine Endopeptidases/chemistry/genetics/*metabolism
MH  - Transfection
MH  - Vero Cells
PMC - PMC2395157
EDAT- 2008/04/11 09:00
MHDA- 2008/08/13 09:00
PMCR- 2008/10/01
CRDT- 2008/04/11 09:00
PHST- 2008/04/11 09:00 [pubmed]
PHST- 2008/08/13 09:00 [medline]
PHST- 2008/04/11 09:00 [entrez]
PHST- 2008/10/01 00:00 [pmc-release]
AID - JVI.02392-07 [pii]
AID - 2392-07 [pii]
AID - 10.1128/JVI.02392-07 [doi]
PST - ppublish
SO  - J Virol. 2008 Jun;82(12):6045-51. doi: 10.1128/JVI.02392-07. Epub 2008 Apr 9.