PMID- 18404520
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 1573-9538 (Print)
IS  - 1573-9546 (Electronic)
IS  - 1573-9538 (Linking)
VI  - 1
IP  - 4
DP  - 2005 Dec
TI  - The priming effect of extracellular UTP on human neutrophils: Role of calcium 
      released from thapsigargin-sensitive intracellular stores.
PG  - 359-68
LID - 10.1007/s11302-005-0039-8 [doi]
AB  - P2Y(2) receptors, which are equally responsive to ATP and UTP, can trigger 
      intracellular signaling events, such as intracellular calcium mobilization and 
      mitogen-activated protein (MAP) kinase phosphorylation in polymorphonuclear 
      leukocytes (PMN). Moreover, extracellular nucleotides have been shown to prime 
      chemoattractant-induced superoxide production. The aim of our study was to 
      investigate the mechanism responsible for the priming effect of extracellular 
      nucleotides on reactive oxygen species (ROS) production induced in human 
      neutrophils by two different chemoattractants: 
      formyl-methionyl-leucyl-phenylalanine (fMLP) and interleukin-8 (IL-8). 
      Nucleotide-induced priming of ROS production was concentration- and 
      time-dependent. When UTP was added to neutrophil suspensions prior to 
      chemoattractant, the increase of the response reached the maximum at 1 min of 
      pre-incubation with the nucleotide. UTP potentiated the phosphorylation of p44/42 
      and p38 MAP kinases induced by chemoattractants, however the P2 receptor-mediated 
      potentiation of ROS production was still detectable in the presence of a SB203580 
      or U0126, supporting the view that MAP kinases do not play a major role in 
      regulating the nucleotide-induced effect. In the presence of thapsigargin, an 
      inhibitor of the ubiquitous sarco-endoplasmic reticulum Ca(2+)-ATPases in 
      mammalian cells, the effect of fMLP was not affected, but UTP-induced priming was 
      abolished, suggesting that the release of calcium from thapsigargin-sensitive 
      intracellular stores is essential for nucleotide-induced priming in human 
      neutrophils.
FAU - Tuluc, Florin
AU  - Tuluc F
AD  - Department of Physiology, Temple University Medical School, Philadelphia, 
      Pennsylvania, USA.
FAU - Bredetean, Ovidiu
AU  - Bredetean O
FAU - Brailoiu, Eugen
AU  - Brailoiu E
FAU - Meshki, John
AU  - Meshki J
FAU - Garcia, Analia
AU  - Garcia A
FAU - Dun, Nae J
AU  - Dun NJ
FAU - Kunapuli, Satya P
AU  - Kunapuli SP
LA  - eng
GR  - R01 HL063933/HL/NHLBI NIH HHS/United States
PT  - Journal Article
DEP - 20051203
PL  - Netherlands
TA  - Purinergic Signal
JT  - Purinergic signalling
JID - 101250499
PMC - PMC2096557
EDAT- 2008/04/12 09:00
MHDA- 2008/04/12 09:01
PMCR- 2005/12/03
CRDT- 2008/04/12 09:00
PHST- 2005/02/22 00:00 [received]
PHST- 2005/06/03 00:00 [accepted]
PHST- 2005/04/26 00:00 [revised]
PHST- 2008/04/12 09:00 [pubmed]
PHST- 2008/04/12 09:01 [medline]
PHST- 2008/04/12 09:00 [entrez]
PHST- 2005/12/03 00:00 [pmc-release]
AID - 20039 [pii]
AID - 10.1007/s11302-005-0039-8 [doi]
PST - ppublish
SO  - Purinergic Signal. 2005 Dec;1(4):359-68. doi: 10.1007/s11302-005-0039-8. Epub 
      2005 Dec 3.