PMID- 18406567
OWN - NLM
STAT- MEDLINE
DCOM- 20080829
LR  - 20131121
IS  - 0887-2333 (Print)
IS  - 0887-2333 (Linking)
VI  - 22
IP  - 5
DP  - 2008 Aug
TI  - Effects of fibrates, anti-inflammatory drugs and antidepressants in the fish 
      hepatoma cell line PLHC-1: cytotoxicity and interactions with cytochrome P450 1A.
PG  - 1128-35
LID - 10.1016/j.tiv.2008.02.020 [doi]
AB  - Effects of 11 pharmaceuticals belonging to three therapeutic classes (lipid 
      regulators from the fibrate group, non-steroidal anti-inflammatory drugs and 
      anti-depressives from the selective serotonin reuptake inhibitors group) were 
      assessed in the fish hepatoma cell line (PLHC-1) by looking at cytotoxicity and 
      interactions with cytochrome P450 1A (CYP1A) function. Among the tested 
      pharmaceuticals, fluoxetine and paroxetine exerted cytotoxic effects, cell 
      viability decreased to 52% and 6% after 24 h of exposure to 20 microM fluoxetine 
      and paroxetine, respectively. The cytotoxicity of both compounds was modulated by 
      cytochrome P450 inhibitors and was dramatically reduced when culture medium was 
      supplemented with reduced glutathione and vitamin E succinate. Additionally, 
      exposure of PLHC-1 cells to some pharmaceuticals led to an early and transient 
      induction of ethoxyresorufin O-deethylase (EROD) activity: bezafibrate and 
      antidepressants induced EROD activity at a concentration of 1 microM whereas 
      clofibrate, ibuprofen and naproxen acted as inducers at a higher concentration 
      (10 microM). These effects might be of toxicological concern since alterations of 
      CYP1A may affect xenobiotic metabolism and toxicity.
FAU - Thibaut, Remi
AU  - Thibaut R
AD  - Environmental Chemistry Department, IIQAB-CSIC, C/Jordi Girona, 18, 08034 
      Barcelona, Spain. rthqam@cid.csic.es
FAU - Porte, Cinta
AU  - Porte C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080304
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Antidepressive Agents)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Water Pollutants, Chemical)
RN  - 0 (Xenobiotics)
RN  - 53PF01Q249 (Clofibric Acid)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/metabolism/*toxicity
MH  - Antidepressive Agents/metabolism/*toxicity
MH  - Carcinoma, Hepatocellular/drug therapy/enzymology
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Clofibric Acid/metabolism/*toxicity
MH  - Cytochrome P-450 CYP1A1/antagonists & inhibitors/*metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Fundulidae
MH  - Hepatocytes/*drug effects/enzymology
MH  - Liver Neoplasms/drug therapy/enzymology
MH  - Water Pollutants, Chemical/*toxicity
MH  - Xenobiotics/toxicity
EDAT- 2008/04/15 09:00
MHDA- 2008/08/30 09:00
CRDT- 2008/04/15 09:00
PHST- 2007/10/09 00:00 [received]
PHST- 2008/01/28 00:00 [revised]
PHST- 2008/02/27 00:00 [accepted]
PHST- 2008/04/15 09:00 [pubmed]
PHST- 2008/08/30 09:00 [medline]
PHST- 2008/04/15 09:00 [entrez]
AID - S0887-2333(08)00072-6 [pii]
AID - 10.1016/j.tiv.2008.02.020 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2008 Aug;22(5):1128-35. doi: 10.1016/j.tiv.2008.02.020. Epub 
      2008 Mar 4.