PMID- 18406869
OWN - NLM
STAT- MEDLINE
DCOM- 20080603
LR  - 20220318
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 182
IP  - 2
DP  - 2008 Apr 15
TI  - Screening for submicroscopic chromosomal rearrangements in Wilms tumor using 
      whole-genome microarrays.
PG  - 84-94
LID - 10.1016/j.cancergencyto.2007.12.015 [doi]
AB  - Wilms tumor is the fourth most common malignancy of childhood; its pathogenesis, 
      however, remains largely unknown. With advancements in cytogenetic techniques, 
      such as array comparative genomic hybridization (aCGH), there is new hope for 
      uncovering small chromosomal microdeletions or microduplications that may 
      contribute to our understanding of Wilms tumor. We performed aCGH on 10 samples 
      of Wilms tumor with normal conventional cytogenetic and chromosomal CGH findings. 
      Array CGH revealed abnormalities in 3 of the 10 samples, including microdeletions 
      (2q37.1, 7q31 approximately q32, and 11q22.3), microduplication (18q21.1), and 
      gains and losses of larger chromosomal areas (1q and 7q gain and loss of 7p, 11q, 
      14q, and 16q). Fluorescence in situ hybridization (FISH) analysis confirmed the 
      abnormalities and revealed the majority of them existed only in a proportion 
      cells (> or =30% of cells). We also performed aCGH on three samples of Wilms 
      tumor with previously identified translocations between chromosomes 1 and 16, to 
      determine the breakpoints. The breakpoints were seen in the pericentromeric 
      regions of both chromosomes. Array CGH is useful for identifying submicroscopic 
      changes in Wilms tumor and is more sensitive for detecting clonal abnormalities 
      than conventional methods.
FAU - Rassekh, Shahrad Rod
AU  - Rassekh SR
AD  - Division of Pediatric Hematology/Oncology/BMT, Department of Pediatrics, British 
      Columbia Children's Hospital, 4480 Oak Street, Vancouver, BC V6R 3M9, Canada. 
      rrassekh@cw.bc.ca <rrassekh@cw.bc.ca>
FAU - Chan, Suzanne
AU  - Chan S
FAU - Harvard, Chansonette
AU  - Harvard C
FAU - Dix, David
AU  - Dix D
FAU - Qiao, Ying
AU  - Qiao Y
FAU - Rajcan-Separovic, Evica
AU  - Rajcan-Separovic E
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - *Chromosome Mapping
MH  - *Genome, Human
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Karyotyping
MH  - Kidney Neoplasms/*genetics/pathology
MH  - Nucleic Acid Hybridization
MH  - *Oligonucleotide Array Sequence Analysis
MH  - Wilms Tumor/*genetics/pathology
EDAT- 2008/04/15 09:00
MHDA- 2008/06/05 09:00
CRDT- 2008/04/15 09:00
PHST- 2007/08/31 00:00 [received]
PHST- 2007/12/19 00:00 [revised]
PHST- 2007/12/28 00:00 [accepted]
PHST- 2008/04/15 09:00 [pubmed]
PHST- 2008/06/05 09:00 [medline]
PHST- 2008/04/15 09:00 [entrez]
AID - S0165-4608(08)00025-3 [pii]
AID - 10.1016/j.cancergencyto.2007.12.015 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2008 Apr 15;182(2):84-94. doi: 
      10.1016/j.cancergencyto.2007.12.015.