PMID- 18406873
OWN - NLM
STAT- MEDLINE
DCOM- 20080603
LR  - 20080414
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 182
IP  - 2
DP  - 2008 Apr 15
TI  - Characterization of 9p21 copy number alterations in human melanoma by 
      fluorescence in situ hybridization.
PG  - 116-21
LID - 10.1016/j.cancergencyto.2008.01.008 [doi]
AB  - Alteration of the CDKN2A (alias p16) tumor suppressor gene, located on 9p21, 
      occurs frequently in familial and sporadic melanomas. Beside CDKN2A, other genes 
      (e.g., CDKN2B, and ARF/p14(ARF), long considered distinct from CDKN2A) on this 
      locus are often deleted or mutated in a large number of tumors including glioma, 
      bladder cancer, and lung cancer. The aim of this study was to evaluate the 
      deletion pattern of the 9p21 locus on a cell-by-cell basis in a large number of 
      melanoma samples using fluorescence in situ hybridization (FISH). In an analysis 
      of 81 primary lesions targeting the 9p21 region and chromosome 9 centromere, high 
      frequency of 9p21 loss (84%) was found. Deletion of 9p21 was present in both 
      early- and late-stage melanomas with similar frequencies. Extra 9p21 copies were 
      rarely seen; they were always associated with polysomy 9 and were observed only 
      in advanced stage melanomas (6 tumors). This FISH study strengthens the 
      hypothesis that the loss of 9p21 occurs frequently in primary melanoma, that the 
      deletion is present in early and late stages of the disease with similar 
      frequency, and that it affects a large extent of the locus.
FAU - Rakosy, Zsuzsa
AU  - Rakosy Z
AD  - Department of Preventive Medicine, Faculty of Public Health, Medical and Health 
      Science Center, University of Debrecen, Kassai str. 26/b, H-4028 Debrecen, 
      Hungary.
FAU - Vizkeleti, Laura
AU  - Vizkeleti L
FAU - Ecsedi, Szilvia
AU  - Ecsedi S
FAU - Begany, Agnes
AU  - Begany A
FAU - Emri, Gabriella
AU  - Emri G
FAU - Adany, Roza
AU  - Adany R
FAU - Balazs, Margit
AU  - Balazs M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Chromosomes, Human, Pair 9/*genetics
MH  - Female
MH  - *Gene Dosage
MH  - Genes, Tumor Suppressor
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Melanoma/*genetics
MH  - Middle Aged
MH  - Skin Neoplasms/*genetics
EDAT- 2008/04/15 09:00
MHDA- 2008/06/05 09:00
CRDT- 2008/04/15 09:00
PHST- 2007/11/20 00:00 [received]
PHST- 2008/01/16 00:00 [revised]
PHST- 2008/01/24 00:00 [accepted]
PHST- 2008/04/15 09:00 [pubmed]
PHST- 2008/06/05 09:00 [medline]
PHST- 2008/04/15 09:00 [entrez]
AID - S0165-4608(08)00030-7 [pii]
AID - 10.1016/j.cancergencyto.2008.01.008 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2008 Apr 15;182(2):116-21. doi: 
      10.1016/j.cancergencyto.2008.01.008.