PMID- 18407055
OWN - NLM
STAT- MEDLINE
DCOM- 20090424
LR  - 20080414
IS  - 1673-8527 (Print)
IS  - 1673-8527 (Linking)
VI  - 35
IP  - 2
DP  - 2008 Feb
TI  - Mutation analyses of integrated HBV genome in hepatitis B patients.
PG  - 85-90
LID - 10.1016/S1673-8527(08)60013-2 [doi]
AB  - Little has been learnt in the last 30 years about detection of HBV genome as well 
      as its mutation analysis between hepatitis B fathers (HBF) and their children. In 
      this study, we used nest polymerase chain reaction (PCR), fluorescence in situ 
      hybridization (FISH), and DNA sequencing analysis, to examine the integrated HBV 
      genome in paraffin-embedded testis tissues, which were taken as samples from HBF, 
      and in peripheral blood mononuclear cells (PBMC) from 74 cases of HBFs and their 
      children who were born after their fathers' HBV infection (caHBF). We found that 
      HBV DNA existed in testis tissues, mainly in the basilar parts of the 
      seminiferous tubules, and also in PBMC of HBF. It was also documented that there 
      were point mutations of poly-loci, insertions and deletions of nucleotides in 
      integrated HBV genomes, and the types of gene mutations in the HBFs were similar 
      to those in caHBF. This study addresses the major types of gene mutations in 
      integrated HBV genome in human patients and also presents reliable evidence of 
      possible genetic transmission of hepatitis B.
FAU - Wang, Peilin
AU  - Wang P
AD  - Laboratory of Medical Genetics, Medical College of Qingdao University, Qingdao, 
      China.
FAU - Wang, Xiuhai
AU  - Wang X
FAU - Cong, Shuying
AU  - Cong S
FAU - Ma, Hongming
AU  - Ma H
FAU - Zhang, Xuecheng
AU  - Zhang X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - J Genet Genomics
JT  - Journal of genetics and genomics = Yi chuan xue bao
JID - 101304616
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Base Sequence
MH  - DNA Mutational Analysis
MH  - Epididymis/metabolism
MH  - Fathers
MH  - Female
MH  - Genome, Viral/*genetics
MH  - Hepatitis B/*genetics/pathology/transmission/*virology
MH  - Hepatitis B virus/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infectious Disease Transmission, Vertical
MH  - Leukocytes, Mononuclear/metabolism
MH  - Male
MH  - *Mutation
MH  - Paraffin Embedding
MH  - Polymerase Chain Reaction
MH  - Testis/metabolism
MH  - Virus Integration/*genetics
EDAT- 2008/04/15 09:00
MHDA- 2009/04/25 09:00
CRDT- 2008/04/15 09:00
PHST- 2007/04/23 00:00 [received]
PHST- 2007/06/04 00:00 [revised]
PHST- 2007/06/04 00:00 [accepted]
PHST- 2008/04/15 09:00 [pubmed]
PHST- 2009/04/25 09:00 [medline]
PHST- 2008/04/15 09:00 [entrez]
AID - S1673-8527(08)60013-2 [pii]
AID - 10.1016/S1673-8527(08)60013-2 [doi]
PST - ppublish
SO  - J Genet Genomics. 2008 Feb;35(2):85-90. doi: 10.1016/S1673-8527(08)60013-2.