PMID- 18408138
OWN - NLM
STAT- MEDLINE
DCOM- 20080708
LR  - 20231213
IS  - 1535-3702 (Print)
IS  - 1535-3699 (Linking)
VI  - 233
IP  - 6
DP  - 2008 Jun
TI  - Generation of mature dendritic cells with unique phenotype and function by in 
      vitro short-term culture of human monocytes in the presence of interleukin-4 and 
      interferon-beta.
PG  - 721-31
LID - 10.3181/0712-RM-333 [doi]
AB  - Dendritic cell (DC)-based immunotherapy has been utilized for the treatment of 
      not only a number of human malignancies but also a select group of infectious 
      diseases. Conventional techniques for the generation and maturation of DCs 
      require 7 days of in vitro culture, which prompted us to seek alternative methods 
      that would hasten the generation of functional human myeloid DCs in vitro. 
      Following the use of a number of cytokines/growth factors, we found that in vitro 
      culture of purified human monocytes, in media containing interleukin (IL)-4, 
      together with interferon (IFN)-beta for 24 hrs, followed by the addition of 
      non-specific antigenic stimuli, such as keyhole limpet hemocyanin (KLH), 
      lipopolysaccharide (LPS), or inactivated human immunodeficiency virus (HIV)-1 
      induced the monocytes to differentiated by 3 days into mature DCs (4B-DCs). These 
      4B-DCs expressed high levels of CD83 and CD11c, as well as markers of immune 
      activation, including CD80 and CD86, human leukocyte antigen (HLA) class I and 
      II, and CD14, but not CD1a. Anti-CD14 blocking antibody interfered with 
      generation of 4B-DCs by LPS, but not by KLH or HIV-1. Interestingly, 4B-DCs, but 
      not conventional DCs generated using macrophage-colony stimulating factor and 
      IL-4 (G4-DCs), expressed OX40 and OX40L. 4B-DCs showed phagocytic activity, and 
      spontaneously produced IL-12 and tumor necrosis factor (TNF)-alpha, but not 
      IL-10. 4B-DCs promoted proliferation of allogeneic naive CD4(+) T cells, 
      producing IFN-(lambda) at lower levels than those stimulated with G4-DCs. 4B-DCs 
      were more potent stimulators of allogeneic bulk CD8(+) T cells producing 
      IFN-(lambda) than G4-DCs. These data indicate that 4B-DCs are unique and may 
      provide a relatively more rapid alternative tool for potential clinical use, as 
      compared with conventional G4-DCs.
FAU - Zhang, Li Feng
AU  - Zhang LF
AD  - Department of Immunology, Graduate School of Medicine, University of the Ryukyus, 
      Uehara 207, Nishihara-cho, Nakagami-gun, Okinawa 903-0215, Japan.
FAU - Okuma, Kazu
AU  - Okuma K
FAU - Tanaka, Reiko
AU  - Tanaka R
FAU - Kodama, Akira
AU  - Kodama A
FAU - Kondo, Kayo
AU  - Kondo K
FAU - Ansari, Aftab A
AU  - Ansari AA
FAU - Tanaka, Yuetsu
AU  - Tanaka Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080411
PL  - Switzerland
TA  - Exp Biol Med (Maywood)
JT  - Experimental biology and medicine (Maywood, N.J.)
JID - 100973463
RN  - 0 (Antigens, CD)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD11c Antigen)
RN  - 0 (Immunoglobulins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 207137-56-2 (Interleukin-4)
RN  - 77238-31-4 (Interferon-beta)
SB  - IM
MH  - Antigens, CD/biosynthesis
MH  - B7-1 Antigen/biosynthesis
MH  - B7-2 Antigen/biosynthesis
MH  - CD11c Antigen/biosynthesis
MH  - Cell Culture Techniques/methods
MH  - Cells, Cultured
MH  - Dendritic Cells/*metabolism
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Immune System
MH  - Immunoglobulins/biosynthesis
MH  - Interferon-beta/*metabolism
MH  - Interleukin-4/*metabolism
MH  - Lipopolysaccharides/metabolism
MH  - Membrane Glycoproteins/biosynthesis
MH  - Monocytes/*metabolism
MH  - Phenotype
MH  - CD83 Antigen
EDAT- 2008/04/15 09:00
MHDA- 2008/07/09 09:00
CRDT- 2008/04/15 09:00
PHST- 2008/04/15 09:00 [pubmed]
PHST- 2008/07/09 09:00 [medline]
PHST- 2008/04/15 09:00 [entrez]
AID - 0712-RM-333 [pii]
AID - 10.3181/0712-RM-333 [doi]
PST - ppublish
SO  - Exp Biol Med (Maywood). 2008 Jun;233(6):721-31. doi: 10.3181/0712-RM-333. Epub 
      2008 Apr 11.