PMID- 18408184
OWN - NLM
STAT- MEDLINE
DCOM- 20080812
LR  - 20171116
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 49
IP  - 8
DP  - 2008 Aug
TI  - Dendritic cells augment choroidal neovascularization.
PG  - 3666-70
LID - 10.1167/iovs.07-1640 [doi]
AB  - PURPOSE: Dendritic cells (DCs) are innate immune cells that have recently been 
      shown to support angiogenesis in tumors, endometriosis, and lymph nodes. A major 
      cause of legal blindness is wet age-related macular degeneration (wet ARMD), 
      wherein abnormal blood vessels grow under the retina, an abnormality also 
      referred to as choroidal neovascularization (CNV). The purpose of the present 
      study was to investigate the role of DCs in the development of CNV. METHODS: 
      Laser photocoagulation was used to induce CNV in C57BL/6J mice. The authors 
      analyzed CNV lesions for the presence of DCs using flow cytometry and 
      immunostaining at designated times. They also analyzed the effects of intravenous 
      DC transplantation on CNV development by measuring the lesion area using confocal 
      microscopy 1 week after laser injury. RESULTS: The authors analyzed CNV lesions 
      for the presence of DCs by flow cytometry and observed that CD11c(+) major 
      histocompatibility complex (MHC) class II(+) DCs transiently infiltrated the CNV 
      lesions, reaching a peak at 2 to 4 days after laser injury. These DCs were mostly 
      immature (CD11c(+) MHCII(low)) and expressed vascular endothelial growth factor 
      receptor 2. Immunostaining of laser-induced CNV lesions confirmed that DCs are 
      located at the sites of newly formed blood vessels. Intravenously injected DCs 
      incorporated into the CNV lesions. However, only immature DCs enhanced CNV size. 
      CONCLUSIONS: These results suggest a role for DCs in promoting angiogenesis and 
      lesion growth in laser-induced CNV. The present data suggest that DCs may 
      represent potential cellular targets for therapeutic intervention in wet ARMD.
FAU - Nakai, Kei
AU  - Nakai K
AD  - Department of Surgery, Vascular Biology Program, Children's Hospital Boston, 
      Boston, Massachusetts 02115, USA.
FAU - Fainaru, Ofer
AU  - Fainaru O
FAU - Bazinet, Lauren
AU  - Bazinet L
FAU - Pakneshan, Pouya
AU  - Pakneshan P
FAU - Benny, Ofra
AU  - Benny O
FAU - Pravda, Elke
AU  - Pravda E
FAU - Folkman, Judah
AU  - Folkman J
FAU - D'Amato, Robert J
AU  - D'Amato RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080411
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (CD11c Antigen)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-2)
SB  - IM
MH  - Animals
MH  - CD11c Antigen/metabolism
MH  - Cell Movement
MH  - Cell Transplantation
MH  - Cells, Cultured
MH  - Choroid/blood supply
MH  - Choroidal Neovascularization/*etiology/*metabolism
MH  - Dendritic Cells/*physiology/transplantation
MH  - Disease Models, Animal
MH  - Endothelium, Vascular/metabolism
MH  - Flow Cytometry
MH  - Histocompatibility Antigens Class II/metabolism
MH  - Laser Coagulation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Confocal
MH  - Vascular Endothelial Growth Factor Receptor-2/metabolism
EDAT- 2008/04/15 09:00
MHDA- 2008/08/13 09:00
CRDT- 2008/04/15 09:00
PHST- 2008/04/15 09:00 [pubmed]
PHST- 2008/08/13 09:00 [medline]
PHST- 2008/04/15 09:00 [entrez]
AID - iovs.07-1640 [pii]
AID - 10.1167/iovs.07-1640 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2008 Aug;49(8):3666-70. doi: 10.1167/iovs.07-1640. 
      Epub 2008 Apr 11.