PMID- 18409380
OWN - NLM
STAT- MEDLINE
DCOM- 20080603
LR  - 20080415
IS  - 0475-1450 (Print)
IS  - 0475-1450 (Linking)
VI  - 39
IP  - 1
DP  - 2008 Jan-Feb
TI  - [Analysis of germ cell populations in ejaculate of men infected with herpes 
      simplex virus].
PG  - 47-57
AB  - Cytological and molecular genetics methods were used to study sperm from patients 
      with sperm infected with herpes simplex virus (HSV) as indicated by virological 
      and immunocytochemical tests. The following methods were used: (1) sperm analysis 
      to evaluate the morphology and functional properties of sperm; (2) fluorescence 
      in situ hybridization (FISH) with DNA probes specific for chromosomes 1, X, and Y 
      to evaluate nondisjunction frequencies of these chromosomes in sperm; and (3) 
      quantitative analysis of immature germ cells in the ejaculate to identify 
      spermatogenic abnormalities. The total sperm count and the count of sperm with 
      normal motility proved similar to the norm. FISH analysis demonstrated no 
      difference in the nondisjunction frequency of chromosomes 1, X, and Y between 
      infertile patients with HSV-infected sperm and fertile donors. Comparative 
      quantitative analysis of immature germ cells from the ejaculate has demonstrated 
      a significant and considerable (threefold) increase in the number of 
      spermatocytes I at the prepachytene stages of prophase I (preleptotene, 
      leptotene, and zygotene) in HSV patients compared to normal donors. At the same 
      time, HSV patients demonstrated a significant decrease in the number of 
      spermatocytes I, a decrease in the proportion of spermatocytes II and spermatids, 
      and a twofold increase in the number of unidentifiable immature germ cells. The 
      data obtained indicate a partial spermatogenic arrest at the early stages of 
      meiotic prophase I in HSV patients, which prompts further research into the 
      cellular mechanisms of abnormal spermatogenesis after viral infection in humans.
FAU - Bocharova, E N
AU  - Bocharova EN
FAU - Kurilo, L F
AU  - Kurilo LF
FAU - Shileiko, L V
AU  - Shileiko LV
FAU - Bragina, E E
AU  - Bragina EE
FAU - Iurov, Iu B
AU  - Iurov IuB
FAU - Vorsanova, S G
AU  - Vorsanova SG
FAU - Iurov, I Iu
AU  - Iurov IIu
FAU - Klimova, R R
AU  - Klimova RR
FAU - Kuchsh, A A
AU  - Kuchsh AA
LA  - rus
PT  - English Abstract
PT  - Journal Article
PL  - Russia (Federation)
TA  - Ontogenez
JT  - Ontogenez
JID - 0341527
SB  - IM
MH  - Chromosomes, Human/metabolism
MH  - Herpes Simplex/metabolism/pathology/*physiopathology
MH  - Humans
MH  - Male
MH  - *Meiosis
MH  - *Simplexvirus
MH  - *Spermatogenesis
MH  - *Spermatozoa/pathology
EDAT- 2008/04/16 09:00
MHDA- 2008/06/05 09:00
CRDT- 2008/04/16 09:00
PHST- 2008/04/16 09:00 [pubmed]
PHST- 2008/06/05 09:00 [medline]
PHST- 2008/04/16 09:00 [entrez]
PST - ppublish
SO  - Ontogenez. 2008 Jan-Feb;39(1):47-57.