PMID- 18412629
OWN - NLM
STAT- MEDLINE
DCOM- 20080701
LR  - 20211110
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Linking)
VI  - 27
IP  - 8
DP  - 2008 Apr
TI  - Interaction between neuropeptide Y (NPY) and brain-derived neurotrophic factor in 
      NPY-mediated neuroprotection against excitotoxicity: a role for microglia.
PG  - 2089-102
LID - 10.1111/j.1460-9568.2008.06172.x [doi]
AB  - The neuroprotective effect of neuropeptide Y (NPY) receptor activation was 
      investigated in organotypic mouse hippocampal slice cultures exposed to the 
      glutamate receptor agonist alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic 
      acid (AMPA). Exposure of 2-week-old slice cultures, derived from 7-day-old 
      C57BL/6 mice, to 8 microm AMPA, for 24 h, induced degeneration of CA1 and CA3 
      pyramidal cells, as measured by cellular uptake of propidium iodide (PI). A 
      significant neuroprotection, with a reduction of PI uptake in CA1 and CA3 
      pyramidal cell layers, was observed after incubation with a Y(2) receptor agonist 
      [NPY(13-36), 300 nm]. This effect was sensitive to the presence of the selective 
      Y(2) receptor antagonist (BIIE0246, 1 microm), but was not affected by addition 
      of TrkB-Fc or by a neutralizing antibody against brain-derived neurotrophic 
      factor (BDNF). Moreover, addition of a Y(1) receptor antagonist (BIBP3226, 1 
      microm) or a NPY-neutralizing antibody helped to disclose a neuroprotective role 
      of endogenous NPY in CA1 region. Cultures exposed to 8 microm AMPA for 24 h, 
      displayed, as measured by an enzyme-linked immunosorbent assay, a significant 
      increase in BDNF. In such cultures there was an up-regulation of neuronal TrkB 
      immunoreactivity, as well as the presence of BDNF-immunoreactive microglial cells 
      at sites of injury. Thus, an increase of AMPA-receptor mediated 
      neurodegeneration, in the mouse hippocampus, was prevented by neuroprotective 
      pathways activated by NPY receptors (Y(1) and Y(2)), which can be affected by 
      BDNF released by microglia and neurons.
FAU - Xapelli, S
AU  - Xapelli S
AD  - Center for Neuroscience and Cell Biology of Coimbra and Institute of 
      Biochemistry, Faculty of Medicine, University of Coimbra, 3004-504 Coimbra, 
      Portugal.
FAU - Bernardino, L
AU  - Bernardino L
FAU - Ferreira, R
AU  - Ferreira R
FAU - Grade, S
AU  - Grade S
FAU - Silva, A P
AU  - Silva AP
FAU - Salgado, J R
AU  - Salgado JR
FAU - Cavadas, C
AU  - Cavadas C
FAU - Grouzmann, E
AU  - Grouzmann E
FAU - Poulsen, F R
AU  - Poulsen FR
FAU - Jakobsen, B
AU  - Jakobsen B
FAU - Oliveira, C R
AU  - Oliveira CR
FAU - Zimmer, J
AU  - Zimmer J
FAU - Malva, J O
AU  - Malva JO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neuropeptide Y)
RN  - 0 (Receptors, AMPA)
RN  - 0 (Receptors, Neuropeptide Y)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Hippocampus/metabolism
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microglia/*metabolism
MH  - Neurons/*metabolism
MH  - Neuropeptide Y/*metabolism
MH  - Organ Culture Techniques
MH  - Receptors, AMPA/metabolism
MH  - Receptors, Neuropeptide Y/antagonists & inhibitors/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2008/04/17 09:00
MHDA- 2008/07/02 09:00
CRDT- 2008/04/17 09:00
PHST- 2008/04/17 09:00 [pubmed]
PHST- 2008/07/02 09:00 [medline]
PHST- 2008/04/17 09:00 [entrez]
AID - EJN6172 [pii]
AID - 10.1111/j.1460-9568.2008.06172.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2008 Apr;27(8):2089-102. doi: 10.1111/j.1460-9568.2008.06172.x.