PMID- 18413121
OWN - NLM
STAT- MEDLINE
DCOM- 20080909
LR  - 20191110
IS  - 0210-5691 (Print)
IS  - 0210-5691 (Linking)
VI  - 32
IP  - 4
DP  - 2008 May
TI  - [Acridine orange staining method in the diagnosis of catheter-related bloodstream 
      infections].
PG  - 168-71
AB  - OBJECTIVE: To study if the utility of acridine orange (AO) staining method on 
      blood extracted through intravenous device (ID) is a reliable method to diagnose 
      catheter-related bloodstream infection (CRB). DESIGN: Prospective and 
      observational study. PATIENTS: Patients with central ID and clinical data 
      consistent with CRB who gave their consent to participate. Patients having 
      another infection site were excluded. INTERVENTION: At the moment of the clinical 
      suspicion of CRB and before removing the ID, blood samples were extracted from 
      peripheral veins and through the ID to be analyzed by AO staining. After 
      extracting the samples, the catheter was removed and sent for microbiological 
      analysis with Linares et al and Maki et al techniques. CRS was defined as 
      development of the same microorganism in the tip of the catheter (endoluminal 
      surface with > or = 10 (3) UFC/ml and/or extraluminal surface > or = 15 UFC/ml) 
      and in the peripheral blood. VARIABLES OF INTEREST: Sensitivity, specificity, 
      negative and positive and negative predictive values and positive likelihood 
      ratios (LR) were calculated for the diagnosis of CRB. RESULTS: A total of 121 
      patients were studied and 4 were diagnosed with CRB: 2 infected with 
      Staphylococcus aureus, 1 with Pseudomonas aeruginosa and 1 with Candida albicans. 
      AO sensitivity was 87.5%, specificity 92.7% and the negative predictive value was 
      99.5%. Positive likelihood ratio was 12.04 and negative LR 0.13. CONCLUSIONS: 
      Although the number of events does not allow for the estimation of the efficacy 
      of AO to diagnose CRB, its high negative predictive value would make it possible 
      to rule out this infectious complication with some degree of safety.
FAU - Quintana, R
AU  - Quintana R
AD  - Segunda Catedra de Clinica Medica, Hospital Escuela Eva Peron, Granadero 
      Baigorria, Argentina.
FAU - Prieto, M F
AU  - Prieto MF
FAU - Bagilet, D H
AU  - Bagilet DH
FAU - Dalman, M C
AU  - Dalman MC
FAU - Gregorini, E
AU  - Gregorini E
LA  - spa
PT  - English Abstract
PT  - Journal Article
TT  - Naranja de acridina para el diagnosticode bacteriemias relacionadas con 
      cateteres.
PL  - Spain
TA  - Med Intensiva
JT  - Medicina intensiva
JID - 9207689
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Bacteremia/*diagnosis/etiology
MH  - Bacteriological Techniques
MH  - Candidiasis/*blood/*microbiology
MH  - Catheters, Indwelling/*adverse effects/microbiology
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Pseudomonas Infections/*blood/*microbiology
MH  - Staphylococcal Infections/*blood/*microbiology
EDAT- 2008/04/17 09:00
MHDA- 2008/09/10 09:00
CRDT- 2008/04/17 09:00
PHST- 2008/04/17 09:00 [pubmed]
PHST- 2008/09/10 09:00 [medline]
PHST- 2008/04/17 09:00 [entrez]
AID - S0210-5691(08)70933-1 [pii]
AID - 10.1016/s0210-5691(08)70933-1 [doi]
PST - ppublish
SO  - Med Intensiva. 2008 May;32(4):168-71. doi: 10.1016/s0210-5691(08)70933-1.