PMID- 1841381
OWN - NLM
STAT- MEDLINE
DCOM- 19921125
LR  - 20041117
IS  - 0261-3166 (Print)
IS  - 0261-3166 (Linking)
IP  - 24
DP  - 1991
TI  - 2'-O-alkyloligoribonucleotides, synthesis and applications in molecular biology.
PG  - 59-62
AB  - Oligo(2'-O-alkylribonucleotides) have been synthesized in which alkyl is methyl, 
      allyl and butyl. The various phosphoramidite monomers of 2'-O-alkyl uridine, 
      cytidine, adenosine, guanosine, inosine and 2,6-diaminopurine riboside have been 
      synthesized from a minimum of key intermediates. Extra protection of the lactam 
      function in uracil and hypoxanthine proves useful. The high stability of the 
      oligomers combined with incorporation of non-radioactive reporter groups such as 
      fluorophores, biotin and 2,4-dinitrophenylamino (DNP) moities renders them as 
      excellent antisense tools for studying RNA processing, for locating and 
      visualising RNA and RNP complexes in cells, for examining splicing complexes by 
      electron microscopy and for the affinity chromatography of RNA or RNP complexes.
FAU - Sproat, B S
AU  - Sproat BS
AD  - European Molecular Biology Laboratory, Heidelberg, FRG.
FAU - Lamond, A I
AU  - Lamond AI
FAU - Garcia, R G
AU  - Garcia RG
FAU - Beijer, B
AU  - Beijer B
FAU - Pieles, U
AU  - Pieles U
FAU - Douglas, M
AU  - Douglas M
FAU - Bohmann, K
AU  - Bohmann K
FAU - Carmo-Fonseco, M
AU  - Carmo-Fonseco M
FAU - Weston, S
AU  - Weston S
FAU - O'Loughlin, S
AU  - O'Loughlin S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Nucleic Acids Symp Ser
JT  - Nucleic acids symposium series
JID - 8007206
RN  - 0 (Antisense Elements (Genetics))
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Oligoribonucleotides)
RN  - 0 (RNA Precursors)
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Small Nuclear)
RN  - 0 (Ribonucleoproteins, Small Nuclear)
RN  - 0 (Ribonucleosides)
SB  - IM
MH  - Antisense Elements (Genetics)
MH  - Base Sequence
MH  - Fluorescent Dyes
MH  - Humans
MH  - Hydrogen Bonding
MH  - Indicators and Reagents
MH  - Oligonucleotide Probes/*chemical synthesis
MH  - Oligoribonucleotides/*chemical synthesis
MH  - RNA Precursors
MH  - RNA Probes/chemical synthesis
MH  - RNA Splicing
MH  - RNA, Small Nuclear/metabolism
MH  - Ribonucleoproteins, Small Nuclear/metabolism
MH  - Ribonucleosides/*chemical synthesis
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
PST - ppublish
SO  - Nucleic Acids Symp Ser. 1991;(24):59-62.