PMID- 18413944 OWN - NLM STAT- MEDLINE DCOM- 20080922 LR - 20091119 IS - 0273-1223 (Print) IS - 0273-1223 (Linking) VI - 57 IP - 6 DP - 2008 TI - Fate and transport of faecal contamination microbial indicators, pathogenic protozoa and Campylobacter in the artificially recharged fractured aquifer of Salento, Italy. PG - 849-56 LID - 10.2166/wst.2008.183 [doi] AB - The study investigates the fate and transport of microorganisms introduced by artificial groundwater recharge at the Nardo fractured aquifer in Salento, Italy. Microbial indicators of faecal contamination, parasitic protozoa (Giardia and Cryptosporidium) and pathogenic bacteria (Campylobacter spp.), were monitored into injected water and groundwater to test the efficiency of the "natural disinfection" into the fractured aquifer. A remarkable decrease of microbial indicators and pathogens was observed suggesting that pathogens removal or inactivation may be possible during water flow in fractured aquifer. The recently described PNA probe CJE195 (Lehtola et al. 2005) was utilised for the rapid and specific detection of Campylobacter spp. by fluorescence in situ hybridization (FISH) after enrichment. FISH results were consistent with those of traditional cultural method (ISO 17995) applied in parallel: time required for Campylobacter identification was reduced of 4 days. CI - Copyright IWA Publishing 2008. FAU - La Mantia, R AU - La Mantia R AD - Water Research Institute CNR, Via Francesco De Blasio, 5, 70100 Bari, Italy. rosanna.lamantia@ba.irsa.cnr.it FAU - Masciopinto, C AU - Masciopinto C FAU - Levantesi, C AU - Levantesi C FAU - Tandoi, V AU - Tandoi V LA - eng PT - Journal Article PL - England TA - Water Sci Technol JT - Water science and technology : a journal of the International Association on Water Pollution Research JID - 9879497 SB - IM MH - Animals MH - Campylobacter/*growth & development MH - Cryptosporidium/genetics/growth & development MH - Environmental Monitoring MH - Eukaryota/genetics/*growth & development MH - Feces/*microbiology/*parasitology MH - Giardia/genetics/growth & development MH - In Situ Hybridization, Fluorescence MH - Italy MH - Water Microbiology EDAT- 2008/04/17 09:00 MHDA- 2008/09/23 09:00 CRDT- 2008/04/17 09:00 PHST- 2008/04/17 09:00 [pubmed] PHST- 2008/09/23 09:00 [medline] PHST- 2008/04/17 09:00 [entrez] AID - 10.2166/wst.2008.183 [doi] PST - ppublish SO - Water Sci Technol. 2008;57(6):849-56. doi: 10.2166/wst.2008.183.