PMID- 18414010
OWN - NLM
STAT- MEDLINE
DCOM- 20080804
LR  - 20240308
IS  - 1016-8478 (Print)
IS  - 1016-8478 (Linking)
VI  - 25
IP  - 2
DP  - 2008 Apr 30
TI  - N-acetylphytosphingosine enhances the radiosensitivity of lung cancer cell line 
      NCI-H460.
PG  - 224-30
AB  - Ceramides are well-known second messengers that induce apoptosis in various kinds 
      of cancer cells, and their effects are closely related to radiation sensitivity. 
      Phytoceramides, the yeast counterparts of the mammalian ceramides, are also 
      reported to induce apoptosis. We investigated the effect of a novel ceramide 
      derivative, N-acetylphytosphingosine (NAPS), on the radiosensitivity of NCI-H460 
      human lung carcinoma cells and its differential cytotoxicity in tumor and normal 
      cells. The combination of NAPS with radiation significantly increased clonogenic 
      cell death and caspase-dependent apoptosis. The combined treatment greatly 
      increased Bax expression and Bid cleavage, but not Bcl-2 expression. However, 
      there was no effect on radiosensitivity and apoptosis in BEAS2B cells, which 
      derive from normal human bronchial epithelium. Cell proliferation and DNA 
      synthesis were significantly inhibited by NAPS in both NCI-H460 and BEAS2B cells, 
      but only the BEAS2B cells recovered by 48h after removal of the NAPS. 
      Furthermore, the NCI-H460 cells underwent more DNA fragmentation than the BEAS2B 
      cells in response to NAPS. Our results indicate that NAPS may be a potential 
      radiosensitizing agent with differential effects on tumor vs. normal cells.
FAU - Han, Youngsoo
AU  - Han Y
AD  - Laboratory of Radiation Sensitization and Protection, Korea Institute of 
      Radiological and Medical Sciences, Seoul 139-706, Korea.
FAU - Kim, Kisung
AU  - Kim K
FAU - Shim, Ji-Young
AU  - Shim JY
FAU - Park, Changsoe
AU  - Park C
FAU - Song, Jie-Young
AU  - Song JY
FAU - Yun, Yeon-Sook
AU  - Yun YS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080331
PL  - United States
TA  - Mol Cells
JT  - Molecules and cells
JID - 9610936
RN  - 0 (Cytostatic Agents)
RN  - 0 (Sphingolipids)
RN  - 0 (bcl-2-Associated X Protein)
RN  - EC 3.4.22.- (Caspases)
RN  - RZ4494XR10 (N-acetylphytosphingosine)
SB  - IM
EIN - Mol Cells. 2009 Jan;27(1):125
MH  - Caspases/metabolism
MH  - Cell Death/drug effects/radiation effects
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects/radiation effects
MH  - Clone Cells
MH  - Cytostatic Agents/pharmacology
MH  - Enzyme Activation/drug effects/radiation effects
MH  - Gamma Rays
MH  - Humans
MH  - Lung Neoplasms/enzymology/*pathology
MH  - Radiation Tolerance/*drug effects
MH  - Sphingolipids/*pharmacology
MH  - bcl-2-Associated X Protein/metabolism
EDAT- 2008/04/17 09:00
MHDA- 2008/08/05 09:00
CRDT- 2008/04/17 09:00
PHST- 2008/04/17 09:00 [pubmed]
PHST- 2008/08/05 09:00 [medline]
PHST- 2008/04/17 09:00 [entrez]
AID - S1016-8478(23)17574-1 [pii]
PST - ppublish
SO  - Mol Cells. 2008 Apr 30;25(2):224-30. Epub 2008 Mar 31.