PMID- 18417357
OWN - NLM
STAT- MEDLINE
DCOM- 20080710
LR  - 20211020
IS  - 1044-0305 (Print)
IS  - 1044-0305 (Linking)
VI  - 19
IP  - 6
DP  - 2008 Jun
TI  - Characterization of phosphate-containing metabolites by calcium adduction and 
      electron capture dissociation.
PG  - 799-808
LID - 10.1016/j.jasms.2008.03.004 [doi]
AB  - Several phosphate-containing metabolites, including nicotinamide adenine 
      dinucleotide (NAD), nicotinamide adenine dinucleotide phosphate (NADP), adenosine 
      5'-diphosphate ribose (ADP-r), adenosine 5'-triphosphate (ATP), and guanosine 
      5'-triphosphate (GTP), have been characterized with electron capture dissociation 
      (ECD) and sustained off-resonance irradiation collision-activated dissociation 
      (SORI-CAD) tandem mass spectrometry (MS/MS) in positive-ion mode. Calcium 
      complexation was used to successfully produce abundant doubly charged cationic 
      precursor ions with or without hydration. This approach enabled application of 
      ECD to acidic metabolites for the first time. Fragmentation pathways observed in 
      ECD and SORI-CAD of calcium-adducted phosphate-containing metabolites were 
      complementary. Unique fragmentation was observed in ECD compared to SORI-CAD 
      MS/MS, including ribose cross-ring cleavage for NAD and NADP, and generation of 
      hydrated product ions, including cross-ring fragments, for hydrated ATP and GTP. 
      A combination of ECD and CAD appears promising for maximizing structural 
      information about metabolites.
FAU - Liu, Haichuan
AU  - Liu H
AD  - Department of Chemistry, University of Michigan, Ann Arbor, Michigan, USA.
FAU - Yoo, Hyun Ju
AU  - Yoo HJ
FAU - Hakansson, Kristina
AU  - Hakansson K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080322
PL  - United States
TA  - J Am Soc Mass Spectrom
JT  - Journal of the American Society for Mass Spectrometry
JID - 9010412
RN  - 0 (Phosphates)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/*chemistry/*metabolism
MH  - Electrons
MH  - Phosphates/*chemistry/*metabolism
MH  - Spectroscopy, Fourier Transform Infrared/*methods
EDAT- 2008/04/18 09:00
MHDA- 2008/07/11 09:00
CRDT- 2008/04/18 09:00
PHST- 2008/01/07 00:00 [received]
PHST- 2008/03/13 00:00 [revised]
PHST- 2008/03/14 00:00 [accepted]
PHST- 2008/04/18 09:00 [pubmed]
PHST- 2008/07/11 09:00 [medline]
PHST- 2008/04/18 09:00 [entrez]
AID - S1044-0305(08)00189-X [pii]
AID - 10.1016/j.jasms.2008.03.004 [doi]
PST - ppublish
SO  - J Am Soc Mass Spectrom. 2008 Jun;19(6):799-808. doi: 10.1016/j.jasms.2008.03.004. 
      Epub 2008 Mar 22.