PMID- 18419598
OWN - NLM
STAT- MEDLINE
DCOM- 20080908
LR  - 20211020
IS  - 1582-1838 (Print)
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Linking)
VI  - 12
IP  - 2
DP  - 2008 Apr
TI  - Correlation between the expression of divalent metal transporter 1 and the 
      content of hypoxia-inducible factor-1 in hypoxic HepG2 cells.
PG  - 569-79
LID - 10.1111/j.1582-4934.2007.00145.x [doi]
AB  - Transferrin and transferrin receptor are two key proteins of iron metabolism that 
      have been identified to be hypoxia-inducible genes. Divalent metal transporter 1 
      (DMT1) is also a key transporter of iron under physiological conditions. In 
      addition, in the 5' regulatory region of human DMT1 (between -412 and -570), 
      there are two motifs (CCAAAGTGCTGGG) that are similar to hypoxia-inducible 
      factor-1 (HIF-1) binding sites. It was therefore speculated that DMT1 might also 
      be a hypoxia-inducible gene. We investigated the effects of hypoxia and 
      hypoxia/re-oxygenation on the expression of DMT1 and the content of HIF-1alpha in 
      HepG2 cells. As we expected, a very similar tendency in the responses of the 
      expression of HIF-1alpha, DMT1+IRE (iron response element) and DMT1-IRE proteins 
      to chemical (CoCl(2)) or physical hypoxia was observed. A highly significant 
      correlation was found between the expression of DMT1 proteins and the contents of 
      HIF-1 in hypoxic cells. After the cells were exposed to hypoxia and subsequent 
      normoxia, no HIF-1alpha could be detected and a significant decrease in DMT1+IRE 
      expression (P<0.05), but not in DMT1-IRE protein (versus the hypoxia group), was 
      observed. The findings implied that the HIF-1 pathway might have a role in the 
      regulation of DMT1+IRE expression during hypoxia.
FAU - Li, Zhu
AU  - Li Z
AD  - Institute for Nautical Medicine and Jiangsu Key Laboratory of Neuroregeneration, 
      Nantong University, Nantong, PR China.
FAU - Lai, Zhang
AU  - Lai Z
FAU - Ya, Ke
AU  - Ya K
FAU - Fang, Du
AU  - Fang D
FAU - Ho, Yung Wing
AU  - Ho YW
FAU - Lei, Yang
AU  - Lei Y
FAU - Ming, Qian Zhong
AU  - Ming QZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (Cation Transport Proteins)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Iron-Regulatory Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (solute carrier family 11- (proton-coupled divalent metal ion transporters), 
      member 2)
RN  - 3G0H8C9362 (Cobalt)
RN  - EVS87XF13W (cobaltous chloride)
SB  - IM
MH  - Carcinoma, Hepatocellular/*metabolism/pathology
MH  - Cation Transport Proteins/genetics/*metabolism
MH  - Cell Hypoxia
MH  - Cell Line, Tumor
MH  - Cell Nucleus/metabolism
MH  - Cell Survival/drug effects
MH  - Cobalt/pharmacology
MH  - Cytoplasm/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Hypoxia-Inducible Factor 1/genetics/*metabolism
MH  - Immunohistochemistry
MH  - Iron-Regulatory Proteins/genetics/*metabolism
MH  - Liver Neoplasms/*metabolism/pathology
MH  - Protein Isoforms/genetics/metabolism
MH  - Time Factors
PMC - PMC3822544
EDAT- 2008/04/19 09:00
MHDA- 2008/09/09 09:00
PMCR- 2008/04/01
CRDT- 2008/04/19 09:00
PHST- 2008/04/19 09:00 [pubmed]
PHST- 2008/09/09 09:00 [medline]
PHST- 2008/04/19 09:00 [entrez]
PHST- 2008/04/01 00:00 [pmc-release]
AID - JCMM145 [pii]
AID - 10.1111/j.1582-4934.2007.00145.x [doi]
PST - ppublish
SO  - J Cell Mol Med. 2008 Apr;12(2):569-79. doi: 10.1111/j.1582-4934.2007.00145.x.