PMID- 18420187
OWN - NLM
STAT- MEDLINE
DCOM- 20080825
LR  - 20161124
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 587
IP  - 1-3
DP  - 2008 Jun 10
TI  - Protective effect of baicalin against lipopolysaccharide/D-galactosamine-induced 
      liver injury in mice by up-regulation of heme oxygenase-1.
PG  - 302-8
LID - 10.1016/j.ejphar.2008.02.081 [doi]
AB  - Baicalin, a traditional anti-inflammatory drug, has been found to protect against 
      liver injury in several experimental animal hepatitis models; however, the 
      mechanisms underlying the hepatoprotective properties of baicalin are poorly 
      understood. In the present study,we investigated the effects of baicalin on the 
      acute liver injury in mice induced by Lipopolysaccharide/D-galactosamine 
      (LPS/D-GalN). Baicalin (50, 150, and 300 mg/kg) was pretreated intraperitoneally 
      (i.p.) at 2, 24, and 48 h respectively before LPS/D-GalN injected in mice. The 
      mortality, hepatic tissue histology, hepatic tissue Tumor necrosis factor-alpha 
      (TNF-alpha) and myeloperoxidase (MPO), plasma levels of TNF-alpha and alanine 
      aminotransferase (ALT) and aspartate aminotransferase (AST) were analyzed. 
      Besides, western blotting analyses of nuclear factor kappa B (NF-kappaB) 
      translocation and Heme oxygenase-1(HO-1) protein expression, as well as HO-1 
      activity were determined. The results showed that baicalin protected against 
      LPS/D-GalN-induced liver injury, including dose-dependent alleviation of 
      mortality and hepatic pathological damage, decrease of ALT/AST release and the 
      rise of MPO. Baicalin reduced nuclear translocation of NF-kappa B, TNF-alpha mRNA 
      and protein levels in hepatic tissues and plasma levels of TNF-alpha induced by 
      LPS/D-GalN. Moreover, baicalin dose-dependently increased HO-1 protein expression 
      and activity. Further, inhibition of HO-1 activity significantly reversed the 
      protective effect of baicalin against LPS/D-GalN-induced liver injury. These 
      results suggest that baicalin can effectively prevent LPS/D-GalN-induced liver 
      injury by inhibition of NF-kappa B activity to reduce TNF-alpha production and 
      the underlying mechanism may be related to up-regulation of HO-1 protein and 
      activity.
FAU - Wan, Jing-Yuan
AU  - Wan JY
AD  - Department of Pharmacology, Chongqing Medical University, Chongqing 400016, PR 
      China. jywan@cqmu.edu.cn
FAU - Gong, Xia
AU  - Gong X
FAU - Zhang, Li
AU  - Zhang L
FAU - Li, Hong-Zhong
AU  - Li HZ
FAU - Zhou, Yu-Fan
AU  - Zhou YF
FAU - Zhou, Qi-Xin
AU  - Zhou QX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080307
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Flavonoids)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 347Q89U4M5 (baicalin)
RN  - 7535-00-4 (Galactosamine)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/*pharmacology
MH  - Blotting, Western
MH  - Chemical and Drug Induced Liver Injury/enzymology/pathology/*prevention & control
MH  - Flavonoids/*pharmacology
MH  - Galactosamine/*antagonists & inhibitors/toxicity
MH  - Heme Oxygenase-1/*metabolism
MH  - Lipopolysaccharides/*antagonists & inhibitors/toxicity
MH  - Liver/enzymology/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Peroxidase/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Up-Regulation/drug effects
EDAT- 2008/04/19 09:00
MHDA- 2008/08/30 09:00
CRDT- 2008/04/19 09:00
PHST- 2007/07/30 00:00 [received]
PHST- 2008/01/26 00:00 [revised]
PHST- 2008/02/28 00:00 [accepted]
PHST- 2008/04/19 09:00 [pubmed]
PHST- 2008/08/30 09:00 [medline]
PHST- 2008/04/19 09:00 [entrez]
AID - S0014-2999(08)00268-9 [pii]
AID - 10.1016/j.ejphar.2008.02.081 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2008 Jun 10;587(1-3):302-8. doi: 10.1016/j.ejphar.2008.02.081. 
      Epub 2008 Mar 7.