PMID- 18421171
OWN - NLM
STAT- MEDLINE
DCOM- 20080725
LR  - 20190608
IS  - 1341-1357 (Print)
IS  - 0007-5124 (Linking)
VI  - 57
IP  - 2
DP  - 2008 Apr
TI  - Inhomogeneity of fecal flora in separately reared laboratory mice, as detected by 
      denaturing gradient gel electrophoresis (DGGE).
PG  - 95-9
AB  - Laboratory mice were divided into 2 groups and introduced to different rooms 
      immediately after being transferred from a mouse farm. Polymerase chain reaction 
      followed by denaturing gradient gel electrophoresis were performed on the V6-V8 
      regions of bacterial 16S rDNA obtained from fecal samples at 0, 1, 2, 3, 4 and 8 
      weeks after the introduction. Binary data were obtained from banding patterns, 
      and Euclidean distances for each week were calculated and analyzed by cluster 
      analysis and non-metric multidimensional scaling. Euclidean distances were 
      significantly higher at weeks 1 and 2 than at week 0 in both groups, although the 
      distances between the 2 groups were significantly higher after week 1 than week 
      0. The distances between the 2 groups were significantly higher than those within 
      each group at weeks 4 and 8. Mice in the 2 groups formed clusters at weeks 2 and 
      3 respectively, and mice were divided into 2 clusters by their respective groups 
      at weeks 4 and 8. Mice in the 2 groups were distributed on opposite sides of the 
      origin on the 2-dimensional plane after week 2. These results suggest that mouse 
      fecal flora changed characteristically, according to the local environment after 
      introduction.
FAU - Fushuku, Seigo
AU  - Fushuku S
AD  - Center for Laboratory Animal Science, National Defense Medical College, 
      Tokorozawa, Saitama, Japan.
FAU - Fukuda, Koichi
AU  - Fukuda K
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Exp Anim
JT  - Experimental animals
JID - 9604830
SB  - IM
MH  - Animal Husbandry
MH  - Animals
MH  - Bacteria/*isolation & purification
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Environment
MH  - Feces/*microbiology
MH  - Housing, Animal
MH  - Intestines/*microbiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Polymerase Chain Reaction
MH  - Time Factors
EDAT- 2008/04/19 09:00
MHDA- 2008/07/26 09:00
CRDT- 2008/04/19 09:00
PHST- 2008/04/19 09:00 [pubmed]
PHST- 2008/07/26 09:00 [medline]
PHST- 2008/04/19 09:00 [entrez]
AID - JST.JSTAGE/expanim/57.95 [pii]
AID - 10.1538/expanim.57.95 [doi]
PST - ppublish
SO  - Exp Anim. 2008 Apr;57(2):95-9. doi: 10.1538/expanim.57.95.