PMID- 18422640
OWN - NLM
STAT- MEDLINE
DCOM- 20081022
LR  - 20131121
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 10
IP  - 7
DP  - 2008 Jul
TI  - Abundance and activity of Chloroflexi-type SAR202 bacterioplankton in the meso- 
      and bathypelagic waters of the (sub)tropical Atlantic.
PG  - 1903-11
LID - 10.1111/j.1462-2920.2008.01627.x [doi]
AB  - The contribution of Chloroflexi-type SAR202 cells to total picoplankton and 
      bacterial abundance and uptake of D- and L-aspartic acids (Asp) was determined in 
      the different meso- and bathypelagic water masses of the (sub)tropical Atlantic 
      (from 35 degrees N to 5 degrees S). Fluorescence in situ hybridization (FISH) 
      revealed that the overall abundance of SAR202 was < or = 1 x 10(3) cells ml(-1) 
      in subsurface waters (100 m layer), increasing in the mesopelagic zone to 3 x 
      10(3) cells ml(-1) and remaining fairly constant down to 4000 m depth. Overall, 
      the percentage of total picoplankton identified as SAR202 increased from < 1% in 
      subsurface waters to 10-20% in the bathypelagic waters. On average, members of 
      the SAR202 cluster accounted for about 30% of the Bacteria in the bathypelagic 
      waters, whereas in the mesopelagic and subsurface waters, SAR202 cells 
      contributed < 5% to total bacterial abundance. The ratio of D-Asp : L-Asp uptake 
      by the bulk picoplankton community increased from the subsurface layer (D-Asp : 
      L-Asp uptake ratio approximately 0.03) to the deeper layers reaching a ratio of 
      approximately 1 at 4000 m depth. Combining FISH with microautoradiography to 
      determine the proportion of SAR202 cells taking up D-Asp versus L-Asp, we found 
      that approximately 30% of the SAR202 cells were taking up L-Asp throughout the 
      water column while D-Asp was essentially not taken up by SAR202. This D-Asp : 
      L-Asp uptake pattern of SAR202 cells is in contrast to that of the bulk bacterial 
      and crenarchaeal community in the bathypelagic ocean, both sustaining a higher 
      fraction of D-Asp-positive cells than L-Asp-positive cells. Thus, although the 
      Chloroflexi-type SAR202 constitutes a major bathypelagic bacterial cluster, it 
      does not contribute to the large fraction of d-Asp utilizing prokaryotic 
      community in the meso- and bathypelagic waters of the North Atlantic, but rather 
      utilizes preferentially L-amino acids.
FAU - Varela, Marta M
AU  - Varela MM
AD  - Department of Biological Oceanography, NOIZ Royal Netherlands Institute for Sea 
      Research, PO BOX 59, 1790 AB Den Burg, the Netherlands.
FAU - van Aken, Hendrik M
AU  - van Aken HM
FAU - Herndl, Gerhard J
AU  - Herndl GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080415
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (DNA, Bacterial)
RN  - 4SR0Q8YD1X (D-Aspartic Acid)
SB  - IM
MH  - Chloroflexi/growth & development/*metabolism
MH  - D-Aspartic Acid/biosynthesis/metabolism
MH  - DNA, Bacterial/analysis
MH  - In Situ Hybridization, Fluorescence
MH  - Marine Biology
MH  - Plankton/growth & development
MH  - Seawater/*microbiology
EDAT- 2008/04/22 09:00
MHDA- 2008/10/23 09:00
CRDT- 2008/04/22 09:00
PHST- 2008/04/22 09:00 [pubmed]
PHST- 2008/10/23 09:00 [medline]
PHST- 2008/04/22 09:00 [entrez]
AID - EMI1627 [pii]
AID - 10.1111/j.1462-2920.2008.01627.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2008 Jul;10(7):1903-11. doi: 10.1111/j.1462-2920.2008.01627.x. 
      Epub 2008 Apr 15.