PMID- 18423376
OWN - NLM
STAT- MEDLINE
DCOM- 20080616
LR  - 20101118
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 371
IP  - 2
DP  - 2008 Jun 27
TI  - Dendritic cell vaccine with mRNA targeted to the proteasome by 
      polyubiquitination.
PG  - 242-6
LID - 10.1016/j.bbrc.2008.04.034 [doi]
AB  - Dendritic cells (DCs) transfected with mRNA encoding tumor-associated antigens 
      (TAAs) can induce tumor-specific T-cell responses. To potentiate this, we 
      transfected mature DCs (mDCs) with mRNA encoding TAA targeted to the proteasome. 
      DCs were generated from bone marrow cells by culture with 20 ng/ml GM-CSF and 
      maturation with 1 microg/ml LPS. These mDCs were then electroporated with 10 
      microg of mRNA. Antigen presentation after electroporation with in vitro 
      transcribed mRNA was compared with mRNA from a construct of the TAA preceded by 
      ubiquitin. Proteasomal targeting of mRNA encoding cotranslationally ubiquitinated 
      antigen was found to enhance intracellular degradation of target protein, and 
      result in more efficient priming and expansion of TAA-specific CD8(+) T-cells. We 
      therefore suggest that RNA-transfected DC vaccine efficacy could be improved by 
      the use of mRNA targeted to the proteasome.
FAU - Hosoi, Akihiro
AU  - Hosoi A
AD  - Department of Immunotherapeutics (Medinet), Graduate School of Medicine, The 
      University of Tokyo, 7-3-1 Hongo, Bunkyo-Ku, Tokyo 113-8655, Japan.
FAU - Takeda, Yayoi
AU  - Takeda Y
FAU - Sakuta, Kazuko
AU  - Sakuta K
FAU - Ueha, Satoshi
AU  - Ueha S
FAU - Kurachi, Makoto
AU  - Kurachi M
FAU - Kimura, Kiminori
AU  - Kimura K
FAU - Maekawa, Ryuji
AU  - Maekawa R
FAU - Kakimi, Kazuhiro
AU  - Kakimi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080428
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Cancer Vaccines)
RN  - 0 (MART-1 Antigen)
RN  - 0 (Mlana protein, mouse)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
SB  - IM
MH  - Animals
MH  - *Antigen Presentation
MH  - Antigens, Neoplasm/analysis/genetics/*metabolism
MH  - Cancer Vaccines/*immunology
MH  - Cell Culture Techniques
MH  - Dendritic Cells/drug effects/*immunology
MH  - Electroporation
MH  - MART-1 Antigen
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Neoplasm Proteins/analysis/genetics/metabolism
MH  - Proteasome Endopeptidase Complex/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Transfection
MH  - *Ubiquitination/genetics
EDAT- 2008/04/22 09:00
MHDA- 2008/06/17 09:00
CRDT- 2008/04/22 09:00
PHST- 2008/04/05 00:00 [received]
PHST- 2008/04/08 00:00 [accepted]
PHST- 2008/04/22 09:00 [pubmed]
PHST- 2008/06/17 09:00 [medline]
PHST- 2008/04/22 09:00 [entrez]
AID - S0006-291X(08)00704-3 [pii]
AID - 10.1016/j.bbrc.2008.04.034 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2008 Jun 27;371(2):242-6. doi: 
      10.1016/j.bbrc.2008.04.034. Epub 2008 Apr 28.