PMID- 18423388
OWN - NLM
STAT- MEDLINE
DCOM- 20080718
LR  - 20131121
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 378
IP  - 1
DP  - 2008 Jul 1
TI  - Electrochemical immunosensor with aptamer-based enzymatic amplification.
PG  - 38-42
LID - 10.1016/j.ab.2008.03.047 [doi]
AB  - An electrochemical immunosensor is reported by using aptamer-based enzymatic 
      amplification with immunoglobulin E (IgE) as the model analyte. In this method, 
      the IgE antibody is covalently immobilized as the capture probe on the gold 
      electrode via a self-assembled monolayer of cysteamine. After the target is 
      captured, the biotinylated anti-IgE aptamer is used as the detection probe. The 
      specific interaction of streptavidin-conjugated alkaline phosphatase to the 
      surface-bound biotinylated detection probe mediates a catalytic reaction of 
      ascorbic acid 2-phosphate substrate to produce a reducing agent ascorbic acid. 
      Then silver ions in the solution can be reduced, leading to the deposition of 
      metallic silver on the electrode surface. The amount of deposited silver, which 
      is determined by the amount of IgE target bound on the electrode surface, can be 
      quantified using the stripping voltammetry. The results obtained demonstrated 
      that the electrochemical immunosensor possesses high specificity and a wide 
      dynamic range with a low detection limit that possibly arises from the 
      combination of the highly specific aptamer and the highly sensitive stripping 
      determination of enzymatically deposited silver.
FAU - Feng, Kejun
AU  - Feng K
AD  - State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry 
      and Chemical Engineering, Hunan University, Changsha 410082, People's Republic of 
      China.
FAU - Kang, Yan
AU  - Kang Y
FAU - Zhao, Jing-Jin
AU  - Zhao JJ
FAU - Liu, Ya-Li
AU  - Liu YL
FAU - Jiang, Jian-Hui
AU  - Jiang JH
FAU - Shen, Guo-Li
AU  - Shen GL
FAU - Yu, Ru-Qin
AU  - Yu RQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080408
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Enzymes, Immobilized)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 6SO6U10H04 (Biotin)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - I38ZP9992A (Magnesium)
SB  - IM
MH  - Alkaline Phosphatase/*metabolism
MH  - Aptamers, Nucleotide/*metabolism
MH  - Biosensing Techniques/*methods
MH  - Biotin/metabolism
MH  - Electrochemistry
MH  - Enzymes, Immobilized/*metabolism
MH  - Humans
MH  - Immunoassay/*methods
MH  - Immunoglobulin E/analysis/chemistry/immunology
MH  - Indicator Dilution Techniques
MH  - Magnesium
EDAT- 2008/04/22 09:00
MHDA- 2008/07/19 09:00
CRDT- 2008/04/22 09:00
PHST- 2008/01/15 00:00 [received]
PHST- 2008/03/21 00:00 [revised]
PHST- 2008/03/28 00:00 [accepted]
PHST- 2008/04/22 09:00 [pubmed]
PHST- 2008/07/19 09:00 [medline]
PHST- 2008/04/22 09:00 [entrez]
AID - S0003-2697(08)00191-7 [pii]
AID - 10.1016/j.ab.2008.03.047 [doi]
PST - ppublish
SO  - Anal Biochem. 2008 Jul 1;378(1):38-42. doi: 10.1016/j.ab.2008.03.047. Epub 2008 
      Apr 8.