PMID- 18423391
OWN - NLM
STAT- MEDLINE
DCOM- 20080729
LR  - 20161126
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1777
IP  - 6
DP  - 2008 Jun
TI  - Distinct organization of energy metabolism in HL-1 cardiac cell line and 
      cardiomyocytes.
PG  - 514-24
LID - 10.1016/j.bbabio.2008.03.019 [doi]
AB  - Expression and function of creatine kinase (CK), adenylate kinase (AK) and 
      hexokinase (HK) isoforms in relation to their roles in regulation of oxidative 
      phosphorylation (OXPHOS) and intracellular energy transfer were assessed in 
      beating (B) and non-beating (NB) cardiac HL-l cell lines and adult rat 
      cardiomyocytes or myocardium. In both types of HL-1 cells, the AK2, CKB, HK1 and 
      HK2 genes were expressed at higher levels than the CKM, CKMT2 and AK1 genes. 
      Contrary to the saponin-permeabilized cardiomyocytes the OXPHOS was coupled to 
      mitochondrial AK and HK but not to mitochondrial CK, and neither direct transfer 
      of adenine nucleotides between CaMgATPases and mitochondria nor functional 
      coupling between CK-MM and CaMgATPases was observed in permeabilized HL-1 cells. 
      The HL-1 cells also exhibited deficient complex I of the respiratory chain. In 
      conclusion, contrary to cardiomyocytes where mitochondria and CaMgATPases are 
      organized into tight complexes which ensure effective energy transfer and 
      feedback signaling between these structures via specialized pathways mediated by 
      CK and AK isoforms and direct adenine nucleotide channeling, these complexes do 
      not exist in HL-1 cells due to less organized energy metabolism.
FAU - Eimre, Margus
AU  - Eimre M
AD  - Department of Pathophysiology, Centre of Molecular and Clinical Medicine, Faculty 
      of Medicine, University of Tartu, Tartu, Estonia.
FAU - Paju, Kalju
AU  - Paju K
FAU - Pelloux, Sophie
AU  - Pelloux S
FAU - Beraud, Nathalie
AU  - Beraud N
FAU - Roosimaa, Mart
AU  - Roosimaa M
FAU - Kadaja, Lumme
AU  - Kadaja L
FAU - Gruno, Marju
AU  - Gruno M
FAU - Peet, Nadezhda
AU  - Peet N
FAU - Orlova, Ehte
AU  - Orlova E
FAU - Remmelkoor, Reele
AU  - Remmelkoor R
FAU - Piirsoo, Andres
AU  - Piirsoo A
FAU - Saks, Valdur
AU  - Saks V
FAU - Seppet, Enn
AU  - Seppet E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080329
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Isoenzymes)
RN  - 0 (Muscle Proteins)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Isoenzymes/metabolism
MH  - Male
MH  - Mitochondria, Heart/*enzymology
MH  - Muscle Proteins/*metabolism
MH  - Myocardium/*enzymology
MH  - Myocytes, Cardiac/*enzymology
MH  - *Oxidative Phosphorylation
MH  - Rats
MH  - Rats, Wistar
EDAT- 2008/04/22 09:00
MHDA- 2008/07/30 09:00
CRDT- 2008/04/22 09:00
PHST- 2008/01/26 00:00 [received]
PHST- 2008/03/15 00:00 [revised]
PHST- 2008/03/18 00:00 [accepted]
PHST- 2008/04/22 09:00 [pubmed]
PHST- 2008/07/30 09:00 [medline]
PHST- 2008/04/22 09:00 [entrez]
AID - S0005-2728(08)00062-5 [pii]
AID - 10.1016/j.bbabio.2008.03.019 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2008 Jun;1777(6):514-24. doi: 10.1016/j.bbabio.2008.03.019. 
      Epub 2008 Mar 29.