PMID- 18425454
OWN - NLM
STAT- MEDLINE
DCOM- 20080702
LR  - 20231213
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 445
DP  - 2008
TI  - Chaperone-mediated autophagy.
PG  - 227-44
LID - 10.1007/978-1-59745-157-4_15 [doi]
AB  - Chaperone-mediated autophagy (CMA) is the only type of autophagy in mammalian 
      cells able to selectively degrade cytosolic proteins in lysosomes. CMA is 
      maximally activated in response to stressors such as prolonged starvation, 
      exposure to toxic compounds, or oxidative stress. We have found that CMA activity 
      decreases in aging and in some age-related disorders such as Parkinson's disease. 
      Impaired CMA under these conditions may be responsible for the accumulation of 
      damaged proteins inside cells and for their higher vulnerability to stressors. In 
      contrast to other forms of autophagy, where substrates are engulfed or 
      sequestered along with other cytosolic components, CMA substrates are 
      translocated one-by-one across the lysosomal membrane. Changes in the 
      levels/activity of the lysosomal components required for substrate translocation 
      can be used to stimulate CMA activity. However, the most unequivocal method to 
      measure CMA is by directly tracking the translocation of substrate proteins into 
      isolated lysosomes.
FAU - Kaushik, S
AU  - Kaushik S
AD  - Department of Anatomy and Structural Biology, Marion Bessin Liver Research 
      Center, Albert Einstein College of Medicine, New York, NY, USA.
FAU - Cuervo, A M
AU  - Cuervo AM
LA  - eng
GR  - R01 AG021904-06/AG/NIA NIH HHS/United States
GR  - R01 AG021904/AG/NIA NIH HHS/United States
GR  - R37 AG021904/AG/NIA NIH HHS/United States
GR  - P01 AG031782-01A18475/AG/NIA NIH HHS/United States
GR  - R03 AG019834-02/AG/NIA NIH HHS/United States
GR  - AG19834/AG/NIA NIH HHS/United States
GR  - P01 AG031782/AG/NIA NIH HHS/United States
GR  - AG021904/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Lysosomal Membrane Proteins)
RN  - 0 (Molecular Chaperones)
SB  - IM
MH  - Animals
MH  - Autophagy/*physiology
MH  - Fluorescent Antibody Technique, Indirect
MH  - HSP70 Heat-Shock Proteins/metabolism/physiology
MH  - Lysosomal Membrane Proteins/metabolism
MH  - Lysosomes/*metabolism
MH  - Models, Biological
MH  - Molecular Chaperones/metabolism/*physiology
MH  - Protein Transport/physiology
MH  - Rats
MH  - Rats, Wistar
PMC - PMC2658709
MID - NIHMS101353
EDAT- 2008/04/22 09:00
MHDA- 2008/07/03 09:00
PMCR- 2009/03/19
CRDT- 2008/04/22 09:00
PHST- 2008/04/22 09:00 [pubmed]
PHST- 2008/07/03 09:00 [medline]
PHST- 2008/04/22 09:00 [entrez]
PHST- 2009/03/19 00:00 [pmc-release]
AID - 10.1007/978-1-59745-157-4_15 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2008;445:227-44. doi: 10.1007/978-1-59745-157-4_15.