PMID- 18425470
OWN - NLM
STAT- MEDLINE
DCOM- 20080612
LR  - 20091119
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 444
DP  - 2008
TI  - Rapid prenatal aneuploidy screening by fluorescence in situ hybridization (FISH).
PG  - 39-47
LID - 10.1007/978-1-59745-066-9_3 [doi]
AB  - The most common aneuploidies in prenatal diagnostics of the second trimenon are 
      trisomies of chromosomes 13, 18, and 21 and gonosomal abnormalities. To detect 
      these trisomies as quickly as possible after amniocentesis, besides using 
      polymerase chain reaction, fluorescence in situ hybridization (FISH), applying 
      corresponding centromeric or locus-specific probes, is the method of choice. 
      Results of a rapid prenatal aneuploidy screening in uncultured amniocytes by 
      using FISH are available within 24 hr or less. However, care has to be taken 
      against possible pitfalls in connection with the commercially available probe 
      sets and thus interpretation of results in general. Here, we explain how rapid 
      prenatal aneuploidy screening is performed using the Food and Drug 
      Administration-approved Aneu Vysion kit (Abbott/Vysis), and a review is given of 
      drawbacks and opportunities of this method.
FAU - Weise, Anja
AU  - Weise A
AD  - Institute of Human Genetics and Anthropology, Friedrich-Schiller-University Jena, 
      Jena, Germany.
FAU - Liehr, Thomas
AU  - Liehr T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Reagent Kits, Diagnostic)
SB  - IM
MH  - Amniocentesis
MH  - *Aneuploidy
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - *Genetic Testing
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Predictive Value of Tests
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
MH  - Reagent Kits, Diagnostic
MH  - Reproducibility of Results
MH  - Time Factors
EDAT- 2008/04/22 09:00
MHDA- 2008/06/13 09:00
CRDT- 2008/04/22 09:00
PHST- 2008/04/22 09:00 [pubmed]
PHST- 2008/06/13 09:00 [medline]
PHST- 2008/04/22 09:00 [entrez]
AID - 10.1007/978-1-59745-066-9_3 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2008;444:39-47. doi: 10.1007/978-1-59745-066-9_3.