PMID- 18428317
OWN - NLM
STAT- MEDLINE
DCOM- 20080523
LR  - 20161021
IS  - 1934-8258 (Electronic)
IS  - 1934-8258 (Linking)
VI  - Chapter 8
DP  - 2001 May
TI  - Preparation of amniocytes for interphase fluorescence in situ hybridization 
      (FISH).
PG  - Unit 8.9
LID - 10.1002/0471142905.hg0809s05 [doi]
AB  - Although FISH has been used to clarify deletions or structural rearrangements, 
      recent work has focused increasingly on its applications to interphase analysis. 
      This unit describes preparation of uncultured amniotic fluid cells for FISH 
      analysis. Cells are swollen, then slides are prepared using either a 
      cytocentrifuge or standard methods. These are then fixed and permeabilized for 
      subsequent FISH. An Alternate Protocol describes attachment of amniocytes to a 
      glass or plastic surface followed by hypotonic swelling, fixation, and 
      permeabilization for subsequent FISH. Interphase FISH analysis of amniotic fluid 
      cells is also described.
FAU - Schwartz, S
AU  - Schwartz S
AD  - Case Western Reserve University, University Hospitals of Cleveland, Cleveland, 
      Ohio, USA.
FAU - Micale, M A
AU  - Micale MA
FAU - Becker, L
AU  - Becker L
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Curr Protoc Hum Genet
JT  - Current protocols in human genetics
JID - 101287858
SB  - IM
MH  - Amniocentesis
MH  - Amnion/*cytology
MH  - Cell Adhesion
MH  - Cytogenetic Analysis/*methods
MH  - Female
MH  - Genetics, Medical
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/genetics
MH  - Pregnancy
EDAT- 2008/04/23 09:00
MHDA- 2008/05/24 09:00
CRDT- 2008/04/23 09:00
PHST- 2008/04/23 09:00 [pubmed]
PHST- 2008/05/24 09:00 [medline]
PHST- 2008/04/23 09:00 [entrez]
AID - 10.1002/0471142905.hg0809s05 [doi]
PST - ppublish
SO  - Curr Protoc Hum Genet. 2001 May;Chapter 8:Unit 8.9. doi: 
      10.1002/0471142905.hg0809s05.