PMID- 18428417
OWN - NLM
STAT- MEDLINE
DCOM- 20080527
LR  - 20161021
IS  - 1934-8258 (Electronic)
IS  - 1934-8258 (Linking)
VI  - Chapter 8
DP  - 2007 Jan
TI  - Preparation of cells from formalin-fixed, paraffin-embedded tissue for use in 
      fluorescence in situ hybridization (FISH) experiments.
PG  - Unit 8.8
LID - 10.1002/0471142905.hg0808s52 [doi]
AB  - Numerical and structural chromosome abnormalities can be accurately detected in 
      cells from archived tissues using fluorescence in situ hybridization (FISH). This 
      unit describes two common approaches to performing FISH in formalin-fixed, 
      paraffin-embedded tissue. The first approach utilizes 4 to 6 microm tissue 
      sections in cases for which preserving tissue morphology is necessary, and the 
      second involves extraction of intact nuclei from 50 microm tissue sections. To 
      interpret FISH results using 4 to 6 microm sections, an adequate number of nuclei 
      must be evaluated to perform statistical analysis. Evaluation of 30 to 50 nuclei 
      from the single cell suspension generally gives an interpretable result.
FAU - Weremowicz, Stanislawa
AU  - Weremowicz S
AD  - Harvard Medical School, Boston, Massachusetts, USA.
FAU - Schofield, Deborah E
AU  - Schofield DE
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Curr Protoc Hum Genet
JT  - Current protocols in human genetics
JID - 101287858
RN  - 1HG84L3525 (Formaldehyde)
SB  - IM
MH  - Cell Nucleus/ultrastructure
MH  - Cells/*cytology
MH  - Chromosome Aberrations/statistics & numerical data
MH  - Formaldehyde
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Paraffin Embedding
MH  - Tissue Fixation
EDAT- 2008/04/23 09:00
MHDA- 2008/05/28 09:00
CRDT- 2008/04/23 09:00
PHST- 2008/04/23 09:00 [pubmed]
PHST- 2008/05/28 09:00 [medline]
PHST- 2008/04/23 09:00 [entrez]
AID - 10.1002/0471142905.hg0808s52 [doi]
PST - ppublish
SO  - Curr Protoc Hum Genet. 2007 Jan;Chapter 8:Unit 8.8. doi: 
      10.1002/0471142905.hg0808s52.