PMID- 18430030
OWN - NLM
STAT- MEDLINE
DCOM- 20080701
LR  - 20201209
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Linking)
VI  - 27
IP  - 9
DP  - 2008 May
TI  - Differential effects of pro-BDNF on sensory neurons after sciatic nerve 
      transection in neonatal rats.
PG  - 2380-90
LID - 10.1111/j.1460-9568.2008.06215.x [doi]
AB  - Brain-derived neurotrophic factor (BDNF) plays a critical role in the development 
      of the central and peripheral nervous systems, and also in neuronal survival 
      after injury. The actions of BDNF are mediated by its high-affinity receptors 
      TrkB and p75NTR. Recent studies have shown that proneurotrophins bind p75NTR and 
      sortilin with high affinity, and trigger apoptosis of neurons in vitro. As 
      proneurotrophins are a dominant form of gene products in developing and adult 
      animals, it is imperative to understand their physiological functions in animals. 
      Here, we showed differential roles of proBDNF in injured and uninjured sensory 
      neurons. proBDNF, p75NTR and sortilin are highly expressed in dorsal root ganglia 
      (DRG) neurons. Recombinant proBDNF induced a dose-dependent death of PC12 cells 
      and the death activity was completely abolished in the presence of antibodies 
      against the prodomain of BDNF. The exogenous proBDNF enhanced the death of 
      axotomized sensory neurons and the neutralizing antibodies to the prodomain or 
      exogenous sortilin-extracellular domain-Fc fusion molecule reduced the death of 
      axotomized sensory neurons. Interestingly, the treatment of neutralizing antibody 
      in vivo increased the number of sensory neurons in the contralateral DRG. We 
      conclude that proBDNF may induce the death of axotomized sensory neurons and 
      suppress neuronal addition in the intact DRG in neonatal rats, and the 
      suppression of endogenous proBDNF may protect neurons after neurotrauma.
FAU - Fan, Yong-Jun
AU  - Fan YJ
AD  - Department of Human Physiology and Centre for Neuroscience, Flinders University, 
      GPO Box 2100, Adelaide, SA 5001, Australia.
FAU - Wu, Linda Lin-Yan
AU  - Wu LL
FAU - Li, Hong-Yun
AU  - Li HY
FAU - Wang, Yan-Jiang
AU  - Wang YJ
FAU - Zhou, Xin-Fu
AU  - Zhou XF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080422
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Adaptor Proteins, Vesicular Transport)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Protein Precursors)
RN  - 0 (Receptors, Growth Factor)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 0 (Recombinant Proteins)
RN  - 0 (brain-derived neurotrophic factor precursor)
RN  - 136958-07-1 (Ngfr protein, rat)
RN  - Z020Y8WIJ4 (sortilin)
SB  - IM
MH  - Adaptor Proteins, Vesicular Transport
MH  - Animals
MH  - Animals, Newborn
MH  - Apoptosis/physiology
MH  - Axotomy
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Functional Laterality
MH  - Ganglia, Spinal/metabolism
MH  - Immunohistochemistry
MH  - Membrane Glycoproteins/biosynthesis
MH  - Nerve Tissue Proteins/biosynthesis
MH  - Neurons, Afferent/*metabolism/pathology
MH  - Protein Precursors/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Growth Factor
MH  - Receptors, Nerve Growth Factor/biosynthesis
MH  - Recombinant Proteins
MH  - Sciatic Nerve/pathology/*physiology
EDAT- 2008/04/24 09:00
MHDA- 2008/07/02 09:00
CRDT- 2008/04/24 09:00
PHST- 2008/04/24 09:00 [pubmed]
PHST- 2008/07/02 09:00 [medline]
PHST- 2008/04/24 09:00 [entrez]
AID - EJN6215 [pii]
AID - 10.1111/j.1460-9568.2008.06215.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2008 May;27(9):2380-90. doi: 10.1111/j.1460-9568.2008.06215.x. 
      Epub 2008 Apr 22.