PMID- 18431718
OWN - NLM
STAT- MEDLINE
DCOM- 20080604
LR  - 20131121
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 216
IP  - 1
DP  - 2008 Jul
TI  - Overexpression of TRPC3 reduces the content of intracellular calcium stores in 
      HEK-293 cells.
PG  - 245-52
LID - 10.1002/jcp.21396 [doi]
AB  - The mammalian canonical transient receptor channels (TRPCs) are considered to be 
      candidates for store-operated calcium channels (SOCCs). Many studies have 
      addressed how TRPC3 channels are affected by depletion of intracellular calcium 
      stores. Conflicting results have been shown for TRPC3 regarding its function, and 
      this has been linked to its level of expression in various systems. In the 
      present study, we have investigated how overexpression of TRPC3 interferes with 
      the regulation of intracellular calcium stores. We demonstrate that 
      overexpression of TRPC3 reduces the mobilization of calcium in response to 
      stimulation of the cells with thapsigargin (TG) or the G-protein coupled receptor 
      agonist sphingosine-1-phosphate (S1P). Our results indicate that this is the 
      result of the expression of TRPC3 channels in the endoplasmic reticulum (ER), 
      thus depleting ER calcium stores. OAG evoked calcium entry in cells 
      overexpressing TRPC3, indicating that functional TRPC3 channels were also 
      expressed in the plasma membrane. Taken together, our results show that 
      overexpression of the putative SOCC, TRPC3, actually reduces the calcium content 
      of intracellular stores, but does not enhance agonist-evoked or store-dependent 
      calcium entry. Our results may, in part, explain the conflicting results obtained 
      in previous studies on the actions of TRPC3 channels.
CI  - (c) 2008 Wiley-Liss, Inc.
FAU - Lof, Christoffer
AU  - Lof C
AD  - Department of Biology, Abo Akademi University, BioCity, Turku, Finland.
FAU - Blom, Tomas
AU  - Blom T
FAU - Tornquist, Kid
AU  - Tornquist K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (1-oleoyl-2-acetoyl-sn-glycerol)
RN  - 0 (Calcium Channels)
RN  - 0 (Diglycerides)
RN  - 0 (Lysophospholipids)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (TRPC Cation Channels)
RN  - 0 (TRPC3 cation channel)
RN  - 139873-08-8 (Calnexin)
RN  - 26993-30-6 (sphingosine 1-phosphate)
RN  - 67526-95-8 (Thapsigargin)
RN  - NGZ37HRE42 (Sphingosine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Calcium Channels/genetics/*metabolism
MH  - Calnexin/metabolism
MH  - Cell Line
MH  - Diglycerides/metabolism
MH  - Endoplasmic Reticulum/metabolism
MH  - Humans
MH  - Ion Channel Gating
MH  - Lysophospholipids/metabolism
MH  - Patch-Clamp Techniques
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Sphingosine/analogs & derivatives/metabolism
MH  - TRPC Cation Channels/genetics/*metabolism
MH  - Thapsigargin/metabolism
EDAT- 2008/04/24 09:00
MHDA- 2008/06/05 09:00
CRDT- 2008/04/24 09:00
PHST- 2008/04/24 09:00 [pubmed]
PHST- 2008/06/05 09:00 [medline]
PHST- 2008/04/24 09:00 [entrez]
AID - 10.1002/jcp.21396 [doi]
PST - ppublish
SO  - J Cell Physiol. 2008 Jul;216(1):245-52. doi: 10.1002/jcp.21396.