PMID- 18431745
OWN - NLM
STAT- MEDLINE
DCOM- 20080627
LR  - 20220311
IS  - 1097-0290 (Electronic)
IS  - 0006-3592 (Linking)
VI  - 100
IP  - 2
DP  - 2008 Jun 1
TI  - Structure analysis and performance of a microbial community from a contaminated 
      aquifer involved in the complete reductive dechlorination of 
      1,1,2,2-tetrachloroethane to ethene.
PG  - 240-9
LID - 10.1002/bit.21776 [doi]
AB  - An anaerobic microcosm set up with aquifer material from a 
      1,1,2,2-tetrachloroethane (TeCA) contaminated site and amended with butyrate 
      showed a complete TeCA dechlorination to ethene. A structure analysis of the 
      microbial community was performed by fluorescence in situ hybridization (FISH) 
      with already available and on purpose designed probes from sequences retrieved 
      through 16S rDNA clone library construction. FISH was chosen as identification 
      tool to evaluate in situ whether the retrieved sequences belong to primary 
      bacteria responsible for the biodegradative reactions. FISH probes identified up 
      to 80% of total bacteria and revealed the absence or the marginal presence of 
      known TeCA degraders and the abundance of two well-known H(2)-utilizing 
      halorespiring bacteria, Sulfurospirillum (32.4 +/- 8.6% of total bacteria) and 
      Dehalococcoides spp. (14.8 +/- 2.8), thereby providing a strong indication of 
      their involvement in the dechlorination processes. These results were supported 
      by the kinetic and thermodynamic analysis which provided indications that 
      hydrogen was the actual electron donor for TeCA dechlorination. The specific 
      probes, developed in this study, for known dechlorinators (i.e., Geobacter, 
      Dehalobacter, and Sulfurospirillum species) represent a valuable tool for any 
      future in situ bioremediation study as well as a quick and specific investigation 
      tool for tracking their distribution in the field.
FAU - Rossetti, Simona
AU  - Rossetti S
AD  - Water Research Institute, IRSA-CNR, Via Reno 1, 00198 Rome, Italy. 
      rossetti@irsa.cnr.it
FAU - Aulenta, Federico
AU  - Aulenta F
FAU - Majone, Mauro
AU  - Majone M
FAU - Crocetti, Gregory
AU  - Crocetti G
FAU - Tandoi, Valter
AU  - Tandoi V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
RN  - 0 (Ethylenes)
RN  - 0 (Hydrocarbons, Chlorinated)
RN  - 0 (Water Pollutants, Chemical)
RN  - 1L6BI049XV (1,1,2,2-tetrachloroethane)
RN  - 4R7X1O2820 (Chlorine)
RN  - 91GW059KN7 (ethylene)
RN  - L99N5N533T (Ethane)
SB  - IM
MH  - Bacteria, Anaerobic/classification/*isolation & purification/*metabolism
MH  - Biodegradation, Environmental
MH  - Chlorine/*metabolism
MH  - Ethane/*analogs & derivatives/metabolism
MH  - Ethylenes/*metabolism
MH  - Hydrocarbons, Chlorinated/*metabolism
MH  - Species Specificity
MH  - *Water Microbiology
MH  - Water Pollutants, Chemical/isolation & purification/*metabolism
MH  - Water Purification
EDAT- 2008/04/24 09:00
MHDA- 2008/06/28 09:00
CRDT- 2008/04/24 09:00
PHST- 2008/04/24 09:00 [pubmed]
PHST- 2008/06/28 09:00 [medline]
PHST- 2008/04/24 09:00 [entrez]
AID - 10.1002/bit.21776 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2008 Jun 1;100(2):240-9. doi: 10.1002/bit.21776.