PMID- 18432814
OWN - NLM
STAT- MEDLINE
DCOM- 20080715
LR  - 20221222
IS  - 1934-368X (Electronic)
IS  - 1934-3671 (Linking)
VI  - Chapter 6
DP  - 2001 May
TI  - Measurement of interleukin 16.
PG  - 6.23.1-6.23.14
LID - 10.1002/0471142735.im0623s22 [doi]
AB  - Interleukin 16 (IL-16) is a chemoattractant immunomodulatory cytokine that 
      initiates its cellular responses through interaction with membrane-expressed CD4. 
      The protein may be detected by a number of methods; the choice of protocol will 
      depend on the ultimate object of a particular experiment. The first method 
      presented is the use of ELISA to measure IL-16 in cell culture supernatants or 
      biological fluids. For some applications, such as identification of IL-16 in an 
      unknown fluid or medium or direct assessment of its bioactivity, functional 
      assays of IL-16-induced responses may be more appropriate. The chemotactic 
      effects of IL-16 on CD4+ T cells and its specific inhibition may be measured 
      using anti-IL-16 antibodies; the same approach may also be applied to monocytes 
      or eosinophils. Another effect of IL-16 is the induction of CD25, which can be 
      assayed using immunological staining. Finally, cell cycle progression in target 
      cells can be measured by the incorporation of radiolabeled thymidine and 
      confirmed by inhibition with neutralizing antibody.
FAU - Center, David M
AU  - Center DM
AD  - Boston University Medical Center, Boston, Massachusetts.
FAU - Cruikshank, William W
AU  - Cruikshank WW
AD  - Boston University Medical Center, Boston, Massachusetts.
FAU - Parada, Nereida A
AU  - Parada NA
AD  - Boston University Medical Center, Boston, Massachusetts.
FAU - Ryan, Thomas
AU  - Ryan T
AD  - Boston University Medical Center, Boston, Massachusetts.
FAU - Theodore, Arthur C
AU  - Theodore AC
AD  - Boston University Medical Center, Boston, Massachusetts.
FAU - Viglianti, Gregory
AU  - Viglianti G
AD  - Boston University School of Medicine, Boston, Massachusetts.
FAU - Lim, Kaiser G
AU  - Lim KG
AD  - The Beth Israel Hospital, Boston, Massachusetts.
FAU - Weller, Peter F
AU  - Weller PF
AD  - The Beth Israel Hospital, Boston, Massachusetts.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Curr Protoc Immunol
JT  - Current protocols in immunology
JID - 9101651
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Interleukin-16)
RN  - 0 (Interleukin-2 Receptor alpha Subunit)
SB  - IM
MH  - Antibodies, Monoclonal/immunology
MH  - Biological Assay/*methods
MH  - CD4-Positive T-Lymphocytes/*physiology
MH  - Cell Cycle
MH  - Chemotaxis, Leukocyte
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Eosinophils/*metabolism
MH  - Humans
MH  - Interleukin-16/*analysis/immunology
MH  - Interleukin-2 Receptor alpha Subunit/immunology/metabolism
EDAT- 2008/04/25 09:00
MHDA- 2008/07/17 09:00
CRDT- 2008/04/25 09:00
PHST- 2008/04/25 09:00 [pubmed]
PHST- 2008/07/17 09:00 [medline]
PHST- 2008/04/25 09:00 [entrez]
AID - 10.1002/0471142735.im0623s22 [doi]
PST - ppublish
SO  - Curr Protoc Immunol. 2001 May;Chapter 6:6.23.1-6.23.14. doi: 
      10.1002/0471142735.im0623s22.