PMID- 18432951 OWN - NLM STAT- MEDLINE DCOM- 20080530 LR - 20161021 IS - 1934-368X (Electronic) IS - 1934-3671 (Linking) VI - Chapter 22 DP - 2005 Jul TI - Differentiation of peripheral blood monocytes into dendritic cells. PG - Unit 22F.4 LID - 10.1002/0471142735.im22f04s67 [doi] AB - Dendritic cells (DCs) are potent antigen-presenting cells (APC) that are important in the initiation and control of cellular immune responses. Commonly used in T cell-stimulation experiments, DCs are typically "matured" in vitro with microbial products or proinflammatory cytokines, and then loaded with antigens from any number of sources, including peptides, whole proteins, cell lysates, RNA, microbes, or killed tumor cells. This unit presents a simple and commonly used method for the generation of mature human dendritic cells--differentiating them from peripheral blood monocytes. FAU - O'Neill, David W AU - O'Neill DW AD - New York University School of Medicine, New York, New York, USA. FAU - Bhardwaj, Nina AU - Bhardwaj N LA - eng PT - Journal Article PL - United States TA - Curr Protoc Immunol JT - Current protocols in immunology JID - 9101651 SB - IM MH - Cell Differentiation MH - Cells, Cultured MH - Dendritic Cells/*cytology MH - Humans MH - Monocytes/*cytology EDAT- 2008/04/25 09:00 MHDA- 2008/05/31 09:00 CRDT- 2008/04/25 09:00 PHST- 2008/04/25 09:00 [pubmed] PHST- 2008/05/31 09:00 [medline] PHST- 2008/04/25 09:00 [entrez] AID - 10.1002/0471142735.im22f04s67 [doi] PST - ppublish SO - Curr Protoc Immunol. 2005 Jul;Chapter 22:Unit 22F.4. doi: 10.1002/0471142735.im22f04s67.