PMID- 18435703
OWN - NLM
STAT- MEDLINE
DCOM- 20090305
LR  - 20211020
IS  - 0031-8655 (Print)
IS  - 1751-1097 (Electronic)
IS  - 0031-8655 (Linking)
VI  - 84
IP  - 5
DP  - 2008 Sep-Oct
TI  - Immunophototherapy using PDT combined with rapid intratumoral dendritic cell 
      injection.
PG  - 1257-64
LID - 10.1111/j.1751-1097.2008.00356.x [doi]
AB  - The capacity of photodynamic therapy (PDT) to induce localized cell death and 
      tissue damage suggests that when applied to tumors it could create a local depot 
      of tumor-associated antigens, which would be available for uptake and 
      presentation to the immune system, potentially leading to improved tumor control. 
      Dendritic cells (DCs) are the most potent cells for antigen uptake, presentation, 
      and stimulation of the immune system. However, it is unclear whether DCs would 
      retain their viability and functional capacity for the requisite trafficking to 
      draining lymph nodes when adoptively transferred in close temporal and anatomic 
      proximity to the site of PDT-induced cytotoxicity. We conducted studies of 
      combined PDT and adoptive DC therapy, "immunophototherapy," in a female, Fisher 
      344 rat orthotopic mammary tumor model. Using 5-aminolevulinic acid as a 
      pro-drug, we demonstrated kinetically favorable biologic conversion to the 
      photosensitive protoporphyrin IX, appropriate trafficking of syngeneic bone 
      marrow-derived DCs injected into PDT-treated tumors within 15 min of completion 
      of therapy, and improved survival over either modality alone. These data indicate 
      that DCs rapidly administered into the site of PDT retain their viability and 
      functional status, supporting the further evaluation of immunophototherapy 
      strategies.
FAU - Sur, Brandon W
AU  - Sur BW
AD  - Division of Hematology/Oncology, Department of Medicine, School of Medicine, 
      University of California, Irvine, CA, USA.
FAU - Nguyen, Phuong
AU  - Nguyen P
FAU - Sun, Chung-Ho
AU  - Sun CH
FAU - Tromberg, Bruce J
AU  - Tromberg BJ
FAU - Nelson, Edward L
AU  - Nelson EL
LA  - eng
GR  - P30 CA062203/CA/NCI NIH HHS/United States
GR  - P41 EB015890/EB/NIBIB NIH HHS/United States
GR  - P41 RR001192/RR/NCRR NIH HHS/United States
GR  - P41-RR01192/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080423
PL  - United States
TA  - Photochem Photobiol
JT  - Photochemistry and photobiology
JID - 0376425
RN  - 0 (Photosensitizing Agents)
RN  - 88755TAZ87 (Aminolevulinic Acid)
SB  - IM
MH  - Aminolevulinic Acid/pharmacology/*therapeutic use
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Dendritic Cells/*immunology
MH  - Drug Screening Assays, Antitumor
MH  - Injections, Intralesional
MH  - Kinetics
MH  - Neoplasms, Experimental/drug therapy/immunology/*therapy
MH  - *Photochemotherapy
MH  - Photosensitizing Agents/pharmacology/*therapeutic use
MH  - Rats
MH  - Rats, Inbred F344
MH  - Survival Rate
MH  - Time Factors
PMC - PMC3940280
MID - NIHMS417210
EDAT- 2008/04/26 09:00
MHDA- 2009/03/06 09:00
PMCR- 2014/03/03
CRDT- 2008/04/26 09:00
PHST- 2008/04/26 09:00 [pubmed]
PHST- 2009/03/06 09:00 [medline]
PHST- 2008/04/26 09:00 [entrez]
PHST- 2014/03/03 00:00 [pmc-release]
AID - PHP356 [pii]
AID - 10.1111/j.1751-1097.2008.00356.x [doi]
PST - ppublish
SO  - Photochem Photobiol. 2008 Sep-Oct;84(5):1257-64. doi: 
      10.1111/j.1751-1097.2008.00356.x. Epub 2008 Apr 23.