PMID- 18435802
OWN - NLM
STAT- MEDLINE
DCOM- 20080722
LR  - 20211020
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 152
IP  - 3
DP  - 2008 Jun
TI  - Identification of immunoglobulin E (IgE)-binding epitopes and recombinant IgE 
      reactivities of a latex cross-reacting Indian jujube Ziz m 1 allergen.
PG  - 464-71
LID - 10.1111/j.1365-2249.2008.03661.x [doi]
AB  - Ziz m 1 is a major Indian jujube (Zizyphus mauritiana) allergen involved in 
      latex-fruit syndrome, and cDNA of the allergen has been cloned, sequenced and 
      expressed in yeast by our laboratory previously. In this study, we performed an 
      immunoglobulin E (IgE)-binding epitope analysis of Ziz m 1 using overlapping 
      recombinant fragments. Eight overlapping recombinant fragments were generated 
      from the recombinant Ziz m 1 allergen. The fragments were expressed in 
      Escherichia coli and IgE-binding activities were evaluated by sera of 
      latex-Indian jujube-allergic subjects and normal subjects using immunoblotting. 
      Human allergic sera are not able to recognize fragments consisting of amino acid 
      sequences 26-71, 119-280 and 119-291. However, residues at positions 26-199, 
      26-105, 26-86, 119-320 and 238-330 were found relevant in the IgE-binding. Our 
      results indicate that (72)NISGHCSDCTFLGEE(86) and 
      (292)VWNRYYDLKTNYSSSIILEYVNSGTKYLP(320) of Ziz m 1 are the sequences required for 
      human IgE binding. Four corresponding peptides, (72)NISGHCSDCTE(86), 
      (292)VWNRYYDLKT(301), (300)KTNYSSSIILEY(311) and (309)LEYVNSGTKYLP(320), were 
      synthesized, and these peptides reacted with 70%, 100%, 70% and 70% of 10 
      allergic sera tested, as revealed by enzyme-linked immunosorbent assay. 
      Sensitization to (292)VWNRYYDLKT(301) correlated significantly with the presence 
      of allergic symptoms (P < 0.001). These findings will be useful in designing 
      diagnostic and therapeutic approaches, thereby contributing to the development of 
      specific immunotherapy for subjects with latex-fruit syndrome.
FAU - Lee, M F
AU  - Lee MF
AD  - Department of Education and Research, Taichung Veterans General Hospital, 
      Taichung, Taiwan.
FAU - Tsai, J J
AU  - Tsai JJ
FAU - Hwang, G Y
AU  - Hwang GY
FAU - Lin, S J
AU  - Lin SJ
FAU - Chen, Y H
AU  - Chen YH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080424
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Epitopes)
RN  - 0 (Peptide Fragments)
RN  - 0 (Plant Extracts)
RN  - 0 (Plant Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Ziz m 1 protein, Indian jujube)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*immunology
MH  - Antigen-Antibody Reactions
MH  - Antigens, Plant
MH  - Cross Reactions
MH  - Epitopes/*analysis/immunology
MH  - Food Hypersensitivity/*immunology
MH  - Humans
MH  - Immunoglobulin E/*metabolism
MH  - Latex Hypersensitivity/*immunology
MH  - Peptide Fragments/metabolism
MH  - Plant Extracts/immunology
MH  - Plant Proteins/*immunology
MH  - Polymerase Chain Reaction/methods
MH  - Recombinant Proteins/metabolism
MH  - Skin Tests/methods
MH  - Ziziphus/*immunology
PMC - PMC2453215
EDAT- 2008/04/26 09:00
MHDA- 2008/07/23 09:00
PMCR- 2009/06/01
CRDT- 2008/04/26 09:00
PHST- 2008/04/26 09:00 [pubmed]
PHST- 2008/07/23 09:00 [medline]
PHST- 2008/04/26 09:00 [entrez]
PHST- 2009/06/01 00:00 [pmc-release]
AID - CEI3661 [pii]
AID - 10.1111/j.1365-2249.2008.03661.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2008 Jun;152(3):464-71. doi: 10.1111/j.1365-2249.2008.03661.x. 
      Epub 2008 Apr 24.