PMID- 18437407
OWN - NLM
STAT- MEDLINE
DCOM- 20090206
LR  - 20211020
IS  - 0937-4477 (Print)
IS  - 0937-4477 (Linking)
VI  - 265
IP  - 11
DP  - 2008 Nov
TI  - Role of siRNA silencing of MMP-2 gene on invasion and growth of laryngeal 
      squamous cell carcinoma.
PG  - 1385-91
LID - 10.1007/s00405-008-0684-y [doi]
AB  - The purpose of this study was to explore the effects of MMP-2 silencing on the 
      invasion and growth of laryngeal squamous cell carcinoma (LSCC). Hep-2 cells were 
      transfected with MMP-2-RNAi-Lentivirus, and MMP-2 expression and invasive 
      properties of the cells were evaluated. The experimental tumors in the nude mice 
      were intratumorally injected with the same recombinant lentivirus. The inhibition 
      of tumor growth was observed. The expression of MMP-2 protein in MMP-2 siRNA 
      transfected Hep-2 cells was effectively suppressed. Both the viability and 
      invasive ability of Hep-2 cells were significantly inhibited. The average weight 
      and volume of tumor in MMP-2-RNAi-Lentivirus treated group were significantly 
      lower than those in the control group (P < 0.01). The PCNA index was obviously 
      lower in MMP-2 RNAi treated tumors (P < 0.01). In conclusion, MMP-2 silencing by 
      recombinant lentivirus mediated RNA interference can inhibit invasion and growth 
      of LSCC, and MMP-2 might be a potential target for gene therapy in LSCC.
FAU - Sun, Yanan
AU  - Sun Y
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, 150086 Harbin, People's Republic of China.
FAU - Liu, Ming
AU  - Liu M
FAU - Yang, Baofeng
AU  - Yang B
FAU - Li, Baoxin
AU  - Li B
FAU - Lu, Jianguang
AU  - Lu J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080425
PL  - Germany
TA  - Eur Arch Otorhinolaryngol
JT  - European archives of oto-rhino-laryngology : official journal of the European 
      Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the 
      German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
JID - 9002937
RN  - 0 (RNA, Small Interfering)
RN  - 0 (RNA-Induced Silencing Complex)
RN  - EC 3.4.24.24 (MMP2 protein, human)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Disease Progression
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Matrix Metalloproteinase 2/*genetics
MH  - Mice
MH  - Neoplasm Invasiveness/*pathology
MH  - RNA, Small Interfering/*genetics
MH  - RNA-Induced Silencing Complex/*genetics
MH  - Transfection
EDAT- 2008/04/26 09:00
MHDA- 2009/02/07 09:00
CRDT- 2008/04/26 09:00
PHST- 2007/12/14 00:00 [received]
PHST- 2008/04/11 00:00 [accepted]
PHST- 2008/04/26 09:00 [pubmed]
PHST- 2009/02/07 09:00 [medline]
PHST- 2008/04/26 09:00 [entrez]
AID - 10.1007/s00405-008-0684-y [doi]
PST - ppublish
SO  - Eur Arch Otorhinolaryngol. 2008 Nov;265(11):1385-91. doi: 
      10.1007/s00405-008-0684-y. Epub 2008 Apr 25.