PMID- 18437589
OWN - NLM
STAT- MEDLINE
DCOM- 20080722
LR  - 20080425
IS  - 1073-1199 (Print)
IS  - 1073-1199 (Linking)
VI  - 36
IP  - 2
DP  - 2008
TI  - Engineering of rat articular cartilage on porous sponges: effects of tgf-beta 1 
      and microgravity bioreactor culture.
PG  - 123-37
LID - 10.1080/10731190801932116 [doi]
AB  - The objective of this study was to develop an engineered rat hyaline cartilage by 
      culturing articular chondrocytes on three-dimensional (3D) macroporous 
      poly(DL-lactic-co-glycolic acid) (PLGA) sponges under chondrogenic induction and 
      microgravity bioreactor conditions. Experimental groups consisted of 3D static 
      and dynamic cultures, while a single cell monolayer (2D) served as the control. 
      The effect of seeding conditions (static vs. dynamic) on cellularization of the 
      scaffolds was investigated. MTT assay was used to evaluate the number of viable 
      cells in each group at different time points. Formation of a hyaline-like 
      cartilage was evaluated for up to 4 weeks in vitro. While 2D culture resulted in 
      cell sheets with very poor matrix production, 3D culture was in the favor of 
      tissue formation. A higher yield of cell attachment and spatially uniform cell 
      distribution was achieved when dynamic seeding technique was used. Dynamic 
      culture promoted cell growth and infiltration throughout the sponge structure and 
      showed the formation of cartilage tissue, while chondrogenesis appeared 
      attenuated more towards the outer region of the constructs in the static culture 
      group. Medium supplemented with TGF-beta 1 (5 ng/ml) had a positive impact on 
      proteoglycan production as confirmed by histochemical analyses with Alcian blue 
      and Safranin-O stainings. Formation of hyaline-like tissue was demonstrated by 
      immunohistochemistry performed with antibodies against type II collagen and 
      aggrecan. SEM confirmed higher level of cellularization and cartilage tissue 
      formation in bioreactor cultures induced by TGF-beta 1. The data suggest that 
      PLGA sponge inside rotating bioreactor with chondrogenic medium provides an 
      environment that mediates isolated rat chondrocytes to redifferentiate and form 
      hyaline-like rat cartilage, in vitro.
FAU - Emin, Nuray
AU  - Emin N
AD  - Ankara University, Faculty of Science and Biotechnology Institute, Tissue 
      Engineering and Biomaterials Laboratory, Ankara, Turkey.
FAU - Koc, Aysel
AU  - Koc A
FAU - Durkut, Serap
AU  - Durkut S
FAU - Elcin, A Eser
AU  - Elcin AE
FAU - Elcin, Y Murat
AU  - Elcin YM
LA  - eng
PT  - Journal Article
PL  - England
TA  - Artif Cells Blood Substit Immobil Biotechnol
JT  - Artificial cells, blood substitutes, and immobilization biotechnology
JID - 9431307
RN  - 0 (Biocompatible Materials)
RN  - 0 (Collagen Type II)
RN  - 0 (Polymers)
RN  - 0 (Proteoglycans)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials
MH  - Bioreactors
MH  - *Cartilage, Articular/cytology/growth & development/metabolism
MH  - Cell Growth Processes
MH  - Cell Movement/drug effects
MH  - Chondrogenesis/drug effects
MH  - Collagen Type II/biosynthesis/metabolism
MH  - Immunohistochemistry
MH  - Microscopy, Electron, Scanning
MH  - Polymers
MH  - Proteoglycans/biosynthesis/metabolism
MH  - Rats
MH  - Tissue Culture Techniques
MH  - *Tissue Engineering
MH  - *Transforming Growth Factor beta1/pharmacology
MH  - Weightlessness
EDAT- 2008/04/26 09:00
MHDA- 2008/07/23 09:00
CRDT- 2008/04/26 09:00
PHST- 2008/04/26 09:00 [pubmed]
PHST- 2008/07/23 09:00 [medline]
PHST- 2008/04/26 09:00 [entrez]
AID - 792554589 [pii]
AID - 10.1080/10731190801932116 [doi]
PST - ppublish
SO  - Artif Cells Blood Substit Immobil Biotechnol. 2008;36(2):123-37. doi: 
      10.1080/10731190801932116.