PMID- 18438029
OWN - NLM
STAT- MEDLINE
DCOM- 20100617
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 26
IP  - 1
DP  - 2008 Jan
TI  - [Determination of ceftazidime and impurities using high performance liquid 
      chromatography].
PG  - 75-9
AB  - A high performance liquid chromatographic (HPLC) method for the determination of 
      ceftazidime and impurities in ceftazidime drug was developed and verified. An 
      Alltima C18 column (250 mm x 4.6 mm, 5 microm) was used as the analysis column. 
      Acetonitrile and phosphate buffer (22.6 g/L aqueous solution of ammonium 
      dihydrogen phosphate, adjusted to pH 3.9 with 10% (v/v) phosphoric acid) were 
      used as mobile phases with gradient elution at a flow rate of 1.3 mL/min. The 
      column temperature was kept at 35 degrees C, and the detection wavelength was set 
      at 255 nm. Fourteen impurities could be well separated. The assay exhibited a 
      good linearity in the ceftazidime concentration range of 0.267-1069 microg/mL 
      with a correlation coefficient of 1.0000. The limits of the quantitation and 
      qualification of ceftazidime were 3.1 ng and 0.93 ng, respectively. The relative 
      standard deviations (RSDs) of the interday and intraday (n=3) determinations at 
      three concentration levels were 0.72% and 0.91%, respectively. At 4 degrees C ang 
      under darkness, ceftazidime solution was stable for 24 h. The developed method is 
      superior to the counterparts in British and Japanese pharmacopeias in the number 
      of the impurities separated and detected.
FAU - Jiang, Enzhu
AU  - Jiang E
AD  - National Institute for the Control of Pharmaceutical and Biological Products, 
      Beijing 100050, China. thinkerpop@gmail.com
FAU - Hu, Changqin
AU  - Hu C
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Pharmaceutical Preparations)
RN  - 9M416Z9QNR (Ceftazidime)
SB  - IM
MH  - Ceftazidime/*analysis/chemistry
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Hydrogen-Ion Concentration
MH  - Limit of Detection
MH  - Linear Models
MH  - Pharmaceutical Preparations/chemistry
MH  - Quality Control
MH  - Reproducibility of Results
EDAT- 2008/04/29 09:00
MHDA- 2010/06/18 06:00
CRDT- 2008/04/29 09:00
PHST- 2008/04/29 09:00 [pubmed]
PHST- 2010/06/18 06:00 [medline]
PHST- 2008/04/29 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2008 Jan;26(1):75-9.