PMID- 18438442
OWN - NLM
STAT- MEDLINE
DCOM- 20080709
LR  - 20220310
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 51
IP  - 5
DP  - 2008 May
TI  - Cytogenetic evidence of nucleolar dominance in allotetraploid species of 
      Brachypodium.
PG  - 387-91
LID - 10.1139/G08-017 [doi]
AB  - Sequential silver staining and fluorescence in situ hybridization (FISH) were 
      used to establish activity and number of 45S rDNA sites in meristematic root tip 
      cells of 6 ecotypes of allotetraploid (2n = 4x = 30) species of Brachypodium and 
      their putative ancestors, B. distachyon (2n = 2x = 10) and ABR114 (2n = 2x = 20). 
      Using either total nuclear DNA of ABR114 or the ABR1-63-E6 BAC clone from a B. 
      distachyon genomic library as an auxiliary probe, it was possible to distinguish 
      by FISH between the two genomes composing the ecotypes of allotetraploid 
      Brachypodium species and to determine unambiguously the parentage of both 
      dominant and suppressed rRNA genes. Each of the diploid species possessed two 
      rDNA loci, both transcriptionally active. The number of 45S rDNA sites in 6 
      ecotypes of allotetraploid Brachypodium species was always equal to the sum of 
      loci present in their putative diploid parents. Two smaller sites were located in 
      chromosomes corresponding to the ABR114 chromosomal set, and two larger ones in 
      the chromosomes of B. distachyon origin. In all analyzed allotetraploid ecotypes, 
      only rRNA genes belonging to the B. distachyon-like genome were transcriptionally 
      active, while rDNA from the other parental genome was always suppressed. Thus the 
      occurrence of nucleolar dominance in the allotetraploid (2n = 4x = 30) species of 
      Brachypodium is demonstrated for the first time.
FAU - Idziak, Dominika
AU  - Idziak D
AD  - Department of Plant Anatomy and Cytology, Faculty of Biology and Environmental 
      Protection, University of Silesia, Katowice, Poland.
FAU - Hasterok, Robert
AU  - Hasterok R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Cell Nucleolus/*genetics
MH  - Chromosomes, Plant
MH  - *Cytogenetics
MH  - DNA, Plant/analysis
MH  - DNA, Ribosomal/analysis/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Meristem/genetics
MH  - Plant Roots/genetics
MH  - Poaceae/*genetics
MH  - *Polyploidy
MH  - Silver Staining
EDAT- 2008/04/29 09:00
MHDA- 2008/07/10 09:00
CRDT- 2008/04/29 09:00
PHST- 2008/04/29 09:00 [pubmed]
PHST- 2008/07/10 09:00 [medline]
PHST- 2008/04/29 09:00 [entrez]
AID - g08-017 [pii]
AID - 10.1139/G08-017 [doi]
PST - ppublish
SO  - Genome. 2008 May;51(5):387-91. doi: 10.1139/G08-017.