PMID- 18438930
OWN - NLM
STAT- MEDLINE
DCOM- 20080930
LR  - 20181201
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Linking)
VI  - 86
IP  - 10
DP  - 2008 Aug 1
TI  - Brain-derived neurotrophic factor stimulates the neural differentiation of human 
      umbilical cord blood-derived mesenchymal stem cells and survival of 
      differentiated cells through MAPK/ERK and PI3K/Akt-dependent signaling pathways.
PG  - 2168-78
LID - 10.1002/jnr.21669 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) plays an important role in the 
      differentiation, development, and survival of neural stem cells. In this study, 
      we analyzed its effects on the stimulation of human umbilical cord blood-derived 
      mesenchymal stem cells in terms of their potential to differentiate into 
      neuron-like cells, their survival characteristics, and the molecular mechanisms 
      involved. The treatment of cells with neural induction medium (NIM) and BDNF 
      generated more cells that were neuron-like and produced stronger expression of 
      neural-lineage markers than cells treated with NIM and without BDNF. Raf-1 and 
      ERK phosphorylation and p35 expression levels increased significantly in cells 
      treated with both NIM and BDNF. This treatment also effectively blocked cell 
      death following neural induction and increased Akt phosphorylation and Bcl2 
      expression compared with cells treated with NIM without BDNF. Inhibition of ERKs 
      inhibited the BDNF-stimulated up-regulation of p35 and Bcl2. In addition, the 
      inhibition of PI3K abrogated Akt phosphorylation and Bcl2 expression, but not p35 
      expression. Thus, MAPK/ERK-dependent p35 up-regulation and MAPK/ERK-dependent and 
      PI3K/Akt-dependent Bcl2 up-regulation contribute to BDNF-stimulated neural 
      differentiation and to the survival of differentiated cells.
FAU - Lim, Jung Yeon
AU  - Lim JY
AD  - Department of Neurosurgery, The Catholic University of Korea, Seoul Korea.
FAU - Park, Sang In
AU  - Park SI
FAU - Oh, Ji Hyeon
AU  - Oh JH
FAU - Kim, Seong Muk
AU  - Kim SM
FAU - Jeong, Chang Hyun
AU  - Jeong CH
FAU - Jun, Jin Ae
AU  - Jun JA
FAU - Lee, Kwan-Sung
AU  - Lee KS
FAU - Oh, Wonil
AU  - Oh W
FAU - Lee, Jae-Kwon
AU  - Lee JK
FAU - Jeun, Sin-Soo
AU  - Jeun SS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (neuronal Cdk5 activator (p25-p35))
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Apoptosis/physiology
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Differentiation/*physiology
MH  - Cell Survival/physiology
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Fetal Blood/cytology
MH  - Fluorescent Antibody Technique
MH  - Gene Expression/physiology
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Mesenchymal Stem Cells/*cytology/*metabolism
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurons/*cytology/metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Signal Transduction/*physiology
MH  - Up-Regulation
EDAT- 2008/04/29 09:00
MHDA- 2008/10/01 09:00
CRDT- 2008/04/29 09:00
PHST- 2008/04/29 09:00 [pubmed]
PHST- 2008/10/01 09:00 [medline]
PHST- 2008/04/29 09:00 [entrez]
AID - 10.1002/jnr.21669 [doi]
PST - ppublish
SO  - J Neurosci Res. 2008 Aug 1;86(10):2168-78. doi: 10.1002/jnr.21669.