PMID- 18439669
OWN - NLM
STAT- MEDLINE
DCOM- 20081208
LR  - 20220330
IS  - 0109-5641 (Print)
IS  - 0109-5641 (Linking)
VI  - 24
IP  - 11
DP  - 2008 Nov
TI  - In vitro induction of hydrolytic activity in human gingival and pulp fibroblasts 
      by triethylene glycol dimethacrylate and monocyte chemotatic protein-1.
PG  - 1461-7
LID - 10.1016/j.dental.2008.03.006 [doi]
AB  - OBJECTIVE: Some dental resin composite materials leach the monomer triethylene 
      glycol dimethacrylate (TEGDMA), which could contribute to the inflammatory 
      reaction. The purpose of this study was to examine the ability of TEGDMA to alter 
      cytokine/growth factor secretion and enzymatic activity in monocyte derived 
      macrophages (U937 cells), human gingival fibroblasts (HGFs), and human pulp 
      fibroblasts (DPFs). METHODS: A human growth factor/cytokine antibody array was 
      utilized to determine whether TEGDMA alters the expression of cytokines/growth 
      factors from U937 cells, HGFs, and DPFs. To determine if TEGDMA alters the 
      hydrolase activity of U937, DPF, and HGF cells, the hydrolysis of p-nitrophenyl 
      butyrate was utilized in a spectrophotometric assay. RESULTS: TEGDMA exposure 
      induced the expression of monocyte chemotatic protein-1 (MCP-1) from the U937 
      cells. Both the cell lysates and conditioned media from the HGFs showed increased 
      hydrolase activity after exposure to a sublethal concentration of TEGDMA. MCP-1 
      alone increased the hydrolytic activity and the combination of MCP-1 and TEGDMA 
      was additive in HGF conditioned media. The DPFs were also exposed to MCP-1 alone 
      and followed by a sublethal concentration of TEGDMA. The effect of MCP-1 was 
      greater than that of TEGDMA. SIGNIFICANCE: These results demonstrate that TEGDMA 
      induces enzymatic activity and cytokine/growth factor expression in a 
      cell-specific manner.
FAU - Gregson, Karen S
AU  - Gregson KS
AD  - Department of Restorative Dentistry, Indiana University School of Dentistry, 
      Indianapolis, IN 46202, USA. kagregso@iupui.edu
FAU - Terrence O'Neill, J
AU  - Terrence O'Neill J
FAU - Platt, Jeffrey A
AU  - Platt JA
FAU - Jack Windsor, L
AU  - Jack Windsor L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080424
PL  - England
TA  - Dent Mater
JT  - Dental materials : official publication of the Academy of Dental Materials
JID - 8508040
RN  - 0 (Chemokine CCL2)
RN  - 0 (Composite Resins)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Polymethacrylic Acids)
RN  - 14I47YJ5EY (triethylene glycol dimethacrylate)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
MH  - Chemokine CCL2/*biosynthesis/physiology
MH  - Composite Resins/*toxicity
MH  - Culture Media, Conditioned/metabolism
MH  - Dental Pulp/cytology/*drug effects
MH  - Fibroblasts/drug effects/enzymology
MH  - Gingiva/cytology/*drug effects
MH  - Humans
MH  - Hydrolysis
MH  - Polyethylene Glycols/*toxicity
MH  - Polymethacrylic Acids/*toxicity
MH  - U937 Cells/drug effects
EDAT- 2008/04/29 09:00
MHDA- 2008/12/17 09:00
CRDT- 2008/04/29 09:00
PHST- 2007/09/24 00:00 [received]
PHST- 2008/02/28 00:00 [revised]
PHST- 2008/03/08 00:00 [accepted]
PHST- 2008/04/29 09:00 [pubmed]
PHST- 2008/12/17 09:00 [medline]
PHST- 2008/04/29 09:00 [entrez]
AID - S0109-5641(08)00072-9 [pii]
AID - 10.1016/j.dental.2008.03.006 [doi]
PST - ppublish
SO  - Dent Mater. 2008 Nov;24(11):1461-7. doi: 10.1016/j.dental.2008.03.006. Epub 2008 
      Apr 24.