PMID- 18440510
OWN - NLM
STAT- MEDLINE
DCOM- 20081112
LR  - 20211020
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 90
IP  - 3
DP  - 2008 Sep
TI  - Nitric oxide synthase isoforms expression in fibroblasts isolated from human 
      normal peritoneum and adhesion tissues.
PG  - 769-74
LID - 10.1016/j.fertnstert.2007.07.1313 [doi]
AB  - OBJECTIVE: To determine the expression of nitric oxide synthases (NOSs) and their 
      modulation by hypoxia in human peritoneal (NF) and adhesion fibroblasts (ADF). 
      DESIGN: Prospective experimental study. SETTING: University medical center. 
      PATIENT(S): Fibroblasts from peritoneum and adhesion tissues. INTERVENTION(S): 
      Hypoxia and silencing inducible NOS (iNOS) gene expression in fibroblasts. MAIN 
      OUTCOME MEASURE(S): We used reverse-transcriptase polymerase chain reaction to 
      quantify messenger RNA (mRNA) levels of NOS isoforms. Griess assay was used to 
      measure NO levels. RESULT(S): The mRNA copies/mug RNA of neuronal NOS (nNOS) and 
      endothelial NOS (eNOS) were 6.6 x 10(3) in NF, 5.7 x 10(3) in ADF and 7.0 x 10(3) 
      in NF, 6.1 x 10(3) in ADF, respectively. The mRNA copies/mug RNA of iNOS were 
      31.3 x 10(3) in NF and 33.0 x 10(3) in ADF. Hypoxia increased iNOS mRNA 
      copies/mug RNA from 31.3 x 10(3) to 61.3 x 10(3) in NF and from 33.0 x 10(3) to 
      63.9 x 10(3) in ADF, whereas there were no changes in mRNA levels of nNOS and 
      eNOS in NF and ADF. Nitric oxide levels were lower in ADF (0.94 micromol/L) than 
      NF (1.97 micromol/L). Silencing iNOS decreased NO levels in NF (from 1.97 
      micromol/L to 0.41 micromol/L) and in ADF (from 0.94 micromol/L to 0.27 
      micromol/L). CONCLUSION(S): Nitric oxide synthases are differentially expressed 
      in NF and ADF, with iNOS being the most expressed and the main source of NO. 
      Hypoxia was shown to alter the expression of NOSs and NO in NF and ADF.
FAU - Jiang, Zhong L
AU  - Jiang ZL
AD  - Division of Reproductive Endocrinology and Infertility, Department of Obstetrics 
      and Gynecology, The C S Mott Center for Human Growth and Development, Wayne State 
      University School of Medicine, Detroit, Michigan 48201, USA.
FAU - Zhu, Xuping
AU  - Zhu X
FAU - Diamond, Michael P
AU  - Diamond MP
FAU - Abu-Soud, Husam M
AU  - Abu-Soud HM
FAU - Saed, Ghassan M
AU  - Saed GM
LA  - eng
GR  - R01 GM069941/GM/NIGMS NIH HHS/United States
GR  - R01 GM069941-01A3/GM/NIGMS NIH HHS/United States
GR  - 1R01 GM069941-01A3/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20080428
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (Isoenzymes)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
SB  - IM
MH  - Cell Adhesion
MH  - Cell Hypoxia/physiology
MH  - Cells, Cultured
MH  - Female
MH  - Fibroblasts/*enzymology
MH  - Humans
MH  - Isoenzymes/metabolism
MH  - Nitric Oxide/*metabolism
MH  - Nitric Oxide Synthase/*metabolism
MH  - Peritoneum/*enzymology
MH  - Tissue Distribution
PMC - PMC2574816
MID - NIHMS55895
EDAT- 2008/04/29 09:00
MHDA- 2008/11/13 09:00
PMCR- 2008/10/28
CRDT- 2008/04/29 09:00
PHST- 2007/03/23 00:00 [received]
PHST- 2007/07/06 00:00 [revised]
PHST- 2007/07/09 00:00 [accepted]
PHST- 2008/04/29 09:00 [pubmed]
PHST- 2008/11/13 09:00 [medline]
PHST- 2008/04/29 09:00 [entrez]
PHST- 2008/10/28 00:00 [pmc-release]
AID - S0015-0282(07)02950-0 [pii]
AID - 10.1016/j.fertnstert.2007.07.1313 [doi]
PST - ppublish
SO  - Fertil Steril. 2008 Sep;90(3):769-74. doi: 10.1016/j.fertnstert.2007.07.1313. 
      Epub 2008 Apr 28.