PMID- 18440539
OWN - NLM
STAT- MEDLINE
DCOM- 20080805
LR  - 20131121
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1193
IP  - 1-2
DP  - 2008 Jun 6
TI  - Rapid determination of water- and fat-soluble vitamins with microemulsion 
      electrokinetic chromatography.
PG  - 172-7
LID - 10.1016/j.chroma.2008.04.016 [doi]
AB  - A rapid, reliable and reproducible method based on microemulsion electrokinetic 
      chromatography (MEEKC) for simultaneous determination of 13 kinds of water- and 
      fat-soluble vitamins has been developed in this work. A novel microemulsion 
      system consisting of 1.2% (w/w) sodium lauryl sulphate (SDS), 21% (v/v) 
      1-butanol, 18% (v/v) acetonitrile, 0.8% (w/w) n-hexane, 20mM borax buffer (pH 
      8.7) was applied to improve selectivity and efficiency, as well as shorten 
      analysis time. The composition of microemulsion used as the MEEKC running buffer 
      was investigated thoroughly to obtain stable separation medium, as well as the 
      optimum determination conditions. Acetonitrile as the organic solvent modifier, 
      pH of the running buffer and 1-butanol as the co-surfactant played the most 
      important roles for the separation of the fat-soluble vitamins, water-soluble 
      vitamins and stabilization of system, respectively. The 13 water- and fat-soluble 
      vitamins were baseline separated within 30 min. The system was applied to 
      determine water- and fat-soluble vitamins in commercial multivitamin 
      pharmaceutical formulation, good accuracy and precision were obtained with 
      recoveries between 97% and 105%, relative standard derivations (RSDs) less than 
      1.8% except vitamin C, and acceptable quantitative results corresponding to label 
      claim.
FAU - Yin, Changna
AU  - Yin C
AD  - School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, 
      China.
FAU - Cao, Yuhua
AU  - Cao Y
FAU - Ding, Shaodong
AU  - Ding S
FAU - Wang, Yun
AU  - Wang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080415
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Buffers)
RN  - 0 (Emulsions)
RN  - 0 (Fats)
RN  - 0 (Solvents)
RN  - 0 (Vitamins)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - Buffers
MH  - Chromatography, Micellar Electrokinetic Capillary/*methods
MH  - Emulsions
MH  - Fats/*chemistry
MH  - Hydrogen-Ion Concentration
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Solubility
MH  - Solvents
MH  - Vitamins/*analysis
MH  - Water/*chemistry
EDAT- 2008/04/29 09:00
MHDA- 2008/08/06 09:00
CRDT- 2008/04/29 09:00
PHST- 2008/01/24 00:00 [received]
PHST- 2008/03/24 00:00 [revised]
PHST- 2008/04/09 00:00 [accepted]
PHST- 2008/04/29 09:00 [pubmed]
PHST- 2008/08/06 09:00 [medline]
PHST- 2008/04/29 09:00 [entrez]
AID - S0021-9673(08)00668-7 [pii]
AID - 10.1016/j.chroma.2008.04.016 [doi]
PST - ppublish
SO  - J Chromatogr A. 2008 Jun 6;1193(1-2):172-7. doi: 10.1016/j.chroma.2008.04.016. 
      Epub 2008 Apr 15.