PMID- 18445008
OWN - NLM
STAT- MEDLINE
DCOM- 20090220
LR  - 20211020
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Print)
IS  - 0019-2805 (Linking)
VI  - 125
IP  - 3
DP  - 2008 Nov
TI  - Serum lipids regulate dendritic cell CD1 expression and function.
PG  - 289-301
LID - 10.1111/j.1365-2567.2008.02842.x [doi]
AB  - Dendritic cells (DCs) are highly potent antigen-presenting cells (APCs) and play 
      a vital role in stimulating naive T cells. Treatment of human blood monocytes 
      with the cytokines granulocyte-macrophage colony-stimulating factor (GM-CSF) and 
      interleukin (IL)-4 stimulates them to develop into immature dendritic cells 
      (iDCs) in vitro. DCs generated by this pathway have a high capacity to prime and 
      activate resting T cells and prominently express CD1 antigen-presenting molecules 
      on the cell surface. The presence of human serum during the differentiation of 
      iDCs from monocytes inhibits the expression of CD1a, CD1b and CD1c, but not CD1d. 
      Correspondingly, T cells that are restricted by CD1c showed poor responses to DCs 
      that were generated in the presence of human serum, while the responses of 
      CD1d-restricted T cells were enhanced. We chemically fractionated human serum to 
      isolate the bioactive factors that modulate surface expression of CD1 proteins 
      during monocyte to DC differentiation. The human serum components that affected 
      CD1 expression partitioned with polar organic soluble fractions. Lysophosphatidic 
      acid and cardiolipin were identified as lipids present in normal human serum that 
      potently modulate CD1 expression. Control of CD1 expression was mediated at the 
      level of gene transcription and correlated with activation of the peroxisome 
      proliferator-activated receptor (PPAR) nuclear hormone receptors. These findings 
      indicate that the ability of human DCs to present lipid antigens to T cells 
      through expression of CD1 molecules is sensitively regulated by lysophosphatidic 
      acid and cardiolipin in serum, which are ligands that can activate PPAR 
      transcription factors.
FAU - Leslie, David S
AU  - Leslie DS
AD  - Division of Rheumatology, Immunology and Allergy, Department of Medicine, Brigham 
      & Women's Hospital and Harvard Medical School, Boston, MA, USA.
FAU - Dascher, Christopher C
AU  - Dascher CC
FAU - Cembrola, Katherine
AU  - Cembrola K
FAU - Townes, Maria A
AU  - Townes MA
FAU - Hava, David L
AU  - Hava DL
FAU - Hugendubler, Lynne C
AU  - Hugendubler LC
FAU - Mueller, Elisabetta
AU  - Mueller E
FAU - Fox, Lisa
AU  - Fox L
FAU - Roura-Mir, Carme
AU  - Roura-Mir C
FAU - Moody, D Branch
AU  - Moody DB
FAU - Vincent, Michael S
AU  - Vincent MS
FAU - Gumperz, Jenny E
AU  - Gumperz JE
FAU - Illarionov, Petr A
AU  - Illarionov PA
FAU - Besra, Gurdyal S
AU  - Besra GS
FAU - Reynolds, Carol G
AU  - Reynolds CG
FAU - Brenner, Michael B
AU  - Brenner MB
LA  - eng
GR  - G0400421/MRC_/Medical Research Council/United Kingdom
GR  - G9901077/MRC_/Medical Research Council/United Kingdom
GR  - R01 HL071590/HL/NHLBI NIH HHS/United States
GR  - R01 AI049313/AI/NIAID NIH HHS/United States
GR  - K08 AR002171/AR/NIAMS NIH HHS/United States
GR  - K08AR02171/AR/NIAMS NIH HHS/United States
GR  - 07221/Z/03/Z/WT_/Wellcome Trust/United Kingdom
GR  - R56 AI074940/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20080428
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antigens, CD1)
RN  - 0 (Lipids)
RN  - 0 (Peroxisome Proliferator-Activated Receptors)
SB  - IM
MH  - Antigen Presentation/immunology
MH  - Antigens, CD1/*metabolism
MH  - Cell Differentiation/immunology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/*immunology
MH  - Gene Expression
MH  - Humans
MH  - Lipids/*immunology
MH  - Lymphocyte Activation/immunology
MH  - Peroxisome Proliferator-Activated Receptors/biosynthesis/genetics/immunology
MH  - Serum/immunology
MH  - T-Lymphocyte Subsets/immunology
PMC - PMC2669133
EDAT- 2008/05/01 09:00
MHDA- 2009/02/21 09:00
PMCR- 2009/11/01
CRDT- 2008/05/01 09:00
PHST- 2008/05/01 09:00 [pubmed]
PHST- 2009/02/21 09:00 [medline]
PHST- 2008/05/01 09:00 [entrez]
PHST- 2009/11/01 00:00 [pmc-release]
AID - IMM2842 [pii]
AID - 10.1111/j.1365-2567.2008.02842.x [doi]
PST - ppublish
SO  - Immunology. 2008 Nov;125(3):289-301. doi: 10.1111/j.1365-2567.2008.02842.x. Epub 
      2008 Apr 28.