PMID- 18445190
OWN - NLM
STAT- MEDLINE
DCOM- 20080722
LR  - 20080430
IS  - 1398-9995 (Electronic)
IS  - 0105-4538 (Linking)
VI  - 63
IP  - 6
DP  - 2008 Jun
TI  - Characterization of Der p 21, a new important allergen derived from the gut of 
      house dust mites.
PG  - 758-67
LID - 10.1111/j.1398-9995.2008.01647.x [doi]
AB  - BACKGROUND: The house dust mite (HDM) Dermatophagoides pteronyssinus is a major 
      allergen source eliciting allergic asthma. The aim of the study was to identify 
      new important HDM allergens associated with allergic asthma. METHODS: A cDNA 
      coding for a new mite allergen, designated Der p 21, was isolated using 
      immunoglobulin E (IgE) antibodies from patients with allergic asthma out of a D. 
      pteronyssinus expression cDNA library and expressed in Escherichia coli. RESULTS: 
      Circular dichroism analysis of the purified allergen showed that rDer p 21 (14 
      726 Da) is one of the few mite allergens with an alpha-helical secondary 
      structure. The protein exhibited high thermal stability and refolding capacity, 
      and, as determined by small angle X-ray scattering, formed a dimer consisting of 
      two flat triangles. rDer p 21 bound high levels of patients' IgE antibodies and 
      showed high allergenic activity in basophil activation experiments. Rabbit 
      anti-Der p 21 IgG antibodies inhibited mite-allergic patients' IgE binding and 
      allowed the ultrastructural localization of the allergen in the midgut 
      (epithelium, lumen and faeces) of D. pteronyssinus by immunogold electron 
      microscopy. Der p 21 revealed sequence homology with group 5 mite allergens, but 
      IgE and IgG reactivity data and cross-inhibition studies identified it as a new 
      mite allergen. CONCLUSIONS: Der p 21 is a new important mite allergen which is 
      liberated into the environment via faecal particles and hence may be associated 
      with allergic asthma.
FAU - Weghofer, M
AU  - Weghofer M
AD  - Division of Immunopathology, Department of Pathophysiology, Center for Physiology 
      and Pathophysiology, Medical University of Vienna, Vienna, Austria.
FAU - Dall'Antonia, Y
AU  - Dall'Antonia Y
FAU - Grote, M
AU  - Grote M
FAU - Stocklinger, A
AU  - Stocklinger A
FAU - Kneidinger, M
AU  - Kneidinger M
FAU - Balic, N
AU  - Balic N
FAU - Krauth, M-T
AU  - Krauth MT
FAU - Fernandez-Caldas, E
AU  - Fernandez-Caldas E
FAU - Thomas, W R
AU  - Thomas WR
FAU - van Hage, M
AU  - van Hage M
FAU - Vieths, S
AU  - Vieths S
FAU - Spitzauer, S
AU  - Spitzauer S
FAU - Horak, F
AU  - Horak F
FAU - Svergun, D I
AU  - Svergun DI
FAU - Konarev, P V
AU  - Konarev PV
FAU - Valent, P
AU  - Valent P
FAU - Thalhamer, J
AU  - Thalhamer J
FAU - Keller, W
AU  - Keller W
FAU - Valenta, R
AU  - Valenta R
FAU - Vrtala, S
AU  - Vrtala S
LA  - eng
SI  - GENBANK/DQ354124
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Allergens)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (DNA, Complementary)
RN  - 0 (Dust)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*chemistry/genetics/*immunology/isolation & purification
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Dermatophagoides/*chemistry/genetics/*immunology/isolation & 
      purification
MH  - Asthma/*immunology
MH  - Base Sequence
MH  - Basophils/immunology
MH  - Circular Dichroism
MH  - DNA, Complementary
MH  - Dermatophagoides pteronyssinus/*immunology/ultrastructure
MH  - Dust/immunology
MH  - Epithelial Cells/immunology/ultrastructure
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Intestines/immunology/ultrastructure
MH  - Microscopy, Immunoelectron
MH  - Molecular Sequence Data
EDAT- 2008/05/01 09:00
MHDA- 2008/07/23 09:00
CRDT- 2008/05/01 09:00
PHST- 2008/05/01 09:00 [pubmed]
PHST- 2008/07/23 09:00 [medline]
PHST- 2008/05/01 09:00 [entrez]
AID - ALL1647 [pii]
AID - 10.1111/j.1398-9995.2008.01647.x [doi]
PST - ppublish
SO  - Allergy. 2008 Jun;63(6):758-67. doi: 10.1111/j.1398-9995.2008.01647.x.