PMID- 18445478
OWN - NLM
STAT- MEDLINE
DCOM- 20080703
LR  - 20211203
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 371
IP  - 3
DP  - 2008 Jul 4
TI  - Activation of AMP-activated protein kinase by kainic acid mediates brain-derived 
      neurotrophic factor expression through a NF-kappaB dependent mechanism in C6 
      glioma cells.
PG  - 495-500
LID - 10.1016/j.bbrc.2008.04.102 [doi]
AB  - AMP-activated protein kinase (AMPK) is a key regulator of energy homeostasis. 
      Kainic acid (KA), a prototype excitotoxin is known to induce brain-derived 
      neurotrophic factor (BDNF) in brain. In this study, we examined the role of AMPK 
      in KA-induced BDNF expression in C6 glioma cells. We showed that KA and KA 
      receptor agonist induced activation of AMPK and KA-induced AMPK activation was 
      blocked by inhibition of Ca(2+)/calmodulin-dependent protein kinase kinase 
      (CaMKK) beta. We then showed that inhibition of AMPK by compound C, a selective 
      inhibitor of AMPK, or small interfering RNA of AMPKalpha1 blocked KA-induced BDNF 
      mRNA and protein expression. Inhibition of AMPK blocked KA-induced 
      phosphorylation of CaMKII and I kappaB kinase (IKK) in C6 cells. Finally, we 
      showed that inhibition of AMPK reduced DNA binding and transcriptional activation 
      of nuclear factor-kappaB (NF-kappaB) in KA-treated cells. These results suggest 
      that AMPK mediates KA-induced BDNF expression by regulating NF-kappaB activation.
FAU - Yoon, Hana
AU  - Yoon H
AD  - Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee 
      University, Seoul 130-701, Republic of Korea.
FAU - Oh, Young Taek
AU  - Oh YT
FAU - Lee, Jung Yeon
AU  - Lee JY
FAU - Choi, Ji Hyun
AU  - Choi JH
FAU - Lee, Ju Hie
AU  - Lee JH
FAU - Baik, Hyung Hwan
AU  - Baik HH
FAU - Kim, Sung Soo
AU  - Kim SS
FAU - Choe, Wonchae
AU  - Choe W
FAU - Yoon, Kyung-Sik
AU  - Yoon KS
FAU - Ha, Joohun
AU  - Ha J
FAU - Kang, Insug
AU  - Kang I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20080428
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Kainic Acid)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Kinase)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - SIV03811UC (Kainic Acid)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - AMP-Activated Protein Kinases
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Calcium/metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Kinase/metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism
MH  - Cell Line, Tumor
MH  - Enzyme Activation
MH  - Glioma
MH  - Kainic Acid/*pharmacology
MH  - Multienzyme Complexes/antagonists & inhibitors/*metabolism
MH  - NF-kappa B/*metabolism
MH  - Phosphorylation
MH  - Protein Serine-Threonine Kinases/antagonists & inhibitors/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Receptors, Kainic Acid/agonists/metabolism
MH  - Transcriptional Activation
EDAT- 2008/05/01 09:00
MHDA- 2008/07/04 09:00
CRDT- 2008/05/01 09:00
PHST- 2008/03/27 00:00 [received]
PHST- 2008/04/19 00:00 [accepted]
PHST- 2008/05/01 09:00 [pubmed]
PHST- 2008/07/04 09:00 [medline]
PHST- 2008/05/01 09:00 [entrez]
AID - S0006-291X(08)00789-4 [pii]
AID - 10.1016/j.bbrc.2008.04.102 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2008 Jul 4;371(3):495-500. doi: 
      10.1016/j.bbrc.2008.04.102. Epub 2008 Apr 28.